Genotype screening for proliferative embryogenesis and biolistic transformation of short-season soybean genotypes

被引:48
作者
Simmonds, DH [1 ]
Donaldson, PA [1 ]
机构
[1] Agr & Agri Food Canada, Eastern Cereal & Oilseed Res Ctr, Cent Expt Farm, Ottawa, ON K1A 0C6, Canada
关键词
soybean; proliferative; embryogenic; bombardment; transformation;
D O I
10.1007/s002990050760
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Eighteen of 20 short-season soybean (Glycine max (L.) Merrill) genotypes (maturity group 0 and 00) screened for proliferative embryogenic capacity formed secondary globular embryos, at rates of 1-70% of cultured immature cotyledons. Five genotypes produced embryogenic cultures which were proliferative for at least 6 months. Proliferative embryogenic cultures of AC Colibri and X2650-7-2-3 were bombarded using a Bio-Rad PDS-1000/He particle gun. Co-bombardments with plasmid pairs pHygr (encoding a type IV aminoglycoside phosphotransferase; aphIV) and pRD300pat (encoding a phosphinothricin N-acetyltransferase; pat) or pRD300pat and pFF19G (beta-glucuronidase; uidA or gus) resulted, respectively, in 12 hygromycin-selected lines with multiple insertions of aphIV and pat, and two 1-phosphinothricin-selected lines plus three beta-glucuronidase-positive lines recovered without selection. Although fertile plants were recovered from young proliferative cultures, transgenic plants, which were derived from cultures 12-14 months of age, were sterile.
引用
收藏
页码:485 / 490
页数:6
相关论文
共 30 条
[1]   GENOTYPE-SPECIFIC OPTIMIZATION OF PLANT-REGENERATION FROM SOMATIC EMBRYOS OF SOYBEAN [J].
BAILEY, MA ;
BOERMA, HR ;
PARROTT, WA .
PLANT SCIENCE, 1993, 93 (1-2) :117-120
[2]  
BAILEY MA, 1993, IN VITRO CELL DEV-PL, V29P, P102
[3]  
BEVERSDORF WD, 1995, HARVEST GOLD HIST FI
[4]   NONRANDOM CHLOROPLAST SEGREGATION IN NICOTIANA-TABACUM (+) N-RUSTICA SOMATIC HYBRIDS SELECTED BY DUAL NUCLEAR-ENCODED RESISTANCE [J].
DONALDSON, P ;
SPROULE, A ;
BEVIS, E ;
PANDEYA, R ;
KELLER, WA ;
GLEDDIE, S .
THEORETICAL AND APPLIED GENETICS, 1993, 86 (04) :465-473
[5]   Susceptibility to Agrobacterium tumefaciens and cotyledonary node transformation in short-season soybean [J].
Donaldson, PA ;
Simmonds, DH .
PLANT CELL REPORTS, 2000, 19 (05) :478-484
[6]  
Draper J., 1988, PLANT GENETIC TRANSF, P199
[7]   APICAL PROLIFERATION OF EMBRYOGENIC TISSUE OF SOYBEAN [GLYCINE-MAX (L) MERRILL] [J].
FINER, JJ .
PLANT CELL REPORTS, 1988, 7 (04) :238-241
[8]   DEVELOPMENT OF AN EMBRYOGENIC SUSPENSION-CULTURE OF SOYBEAN (GLYCINE-MAX MERRILL) [J].
FINER, JJ ;
NAGASAWA, A .
PLANT CELL TISSUE AND ORGAN CULTURE, 1988, 15 (02) :125-136
[9]  
Finer JJ, 1991, IN VITRO CELL DEV-PL, V27, P175, DOI [DOI 10.1007/BF02632213], DOI 10.1007/BF02632213]
[10]   TRANSGENE INACTIVATION - PLANTS FIGHT BACK [J].
FINNEGAN, J ;
MCELROY, D .
BIO-TECHNOLOGY, 1994, 12 (09) :883-888