Development and Production of Commercial Therapeutic Monoclonal Antibodies in Mammalian Cell Expression Systems: An Overview of the Current Upstream Technologies

被引：53

作者：

Chartrain, Michel ^{[1
]}

Chu, Lily ^{[1
]}

机构：

[1] Merck Res Labs, Dept Bioproc R&D, Rahway, NJ 07065 USA

来源：

CURRENT PHARMACEUTICAL BIOTECHNOLOGY | 2008年 / 9卷 / 06期

关键词：

D O I：

10.2174/138920108786786367

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This article provides an overview of the upstream technologies used in the industrial production of therapeutic monoclonal antibodies (mAbs) based on the cultivation of mammalian cells. More specifically, in a first section, after a short discussion of relevant biochemical characteristics of antibodies, we review the cell lines currently employed in commercial production and the methods of constructing and isolating production clones. This is followed with a review of the most current methods of commercial scale production and their associated technologies. Selected references and short discussions pertaining to emerging and relevant technologies have been embedded throughout the text in order to give a sense of the overall direction the field is taking.

引用

页码：447 / 467

页数：21

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