Nitrilotriacetic acid-modified magnetic nanoparticles as a general agent to bind histidine-tagged proteins

被引:403
作者
Xu, CJ
Xu, KM
Gu, HW
Zhong, XF
Guo, ZH
Zheng, RK
Zhang, XX
Xu, B [1 ]
机构
[1] Hong Kong Univ Sci & Technol, Dept Chem, Hong Kong, Hong Kong, Peoples R China
[2] Hong Kong Univ Sci & Technol, Dept Phys, Hong Kong, Hong Kong, Peoples R China
[3] Hong Kong Univ Sci & Technol, Bioengn Program, Hong Kong, Hong Kong, Peoples R China
关键词
D O I
10.1021/ja031776d
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Using Nα,Nα-bis(carboxymethyl)lysine to react with FePt magnetic nanoparticles, we synthesized the FePt?NTA conjugate, which immobilizes Ni2+ ions and selectively binds to histidine-tagged proteins at concentration as low as 0.5 pM. This simple system serves as a useful alternative to existing protocols for protein separation and also acts as a versatile agent for transporting and anchoring proteins. Copyright © 2004 American Chemical Society.
引用
收藏
页码:3392 / 3393
页数:2
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