Nitrilotriacetic acid-modified magnetic nanoparticles as a general agent to bind histidine-tagged proteins

被引：403

作者：

Xu, CJ

Xu, KM

Gu, HW

Zhong, XF

Guo, ZH

Zheng, RK

Zhang, XX

Xu, B ^{[1
]}

机构：

[1] Hong Kong Univ Sci & Technol, Dept Chem, Hong Kong, Hong Kong, Peoples R China

[2] Hong Kong Univ Sci & Technol, Dept Phys, Hong Kong, Hong Kong, Peoples R China

[3] Hong Kong Univ Sci & Technol, Bioengn Program, Hong Kong, Hong Kong, Peoples R China

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 11期

关键词：

D O I：

10.1021/ja031776d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Using Nα,Nα-bis(carboxymethyl)lysine to react with FePt magnetic nanoparticles, we synthesized the FePt?NTA conjugate, which immobilizes Ni2+ ions and selectively binds to histidine-tagged proteins at concentration as low as 0.5 pM. This simple system serves as a useful alternative to existing protocols for protein separation and also acts as a versatile agent for transporting and anchoring proteins. Copyright © 2004 American Chemical Society.

引用

页码：3392 / 3393

页数：2

共 25 条

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