Activity of Candida rugosa lipase immobilized on γ-Fe2O3 magnetic nanoparticles

被引:510
作者
Dyal, A [1 ]
Loos, K [1 ]
Noto, M [1 ]
Chang, SW [1 ]
Spagnoli, C [1 ]
Shafi, KVPM [1 ]
Ulman, A [1 ]
Cowman, M [1 ]
Gross, RA [1 ]
机构
[1] Polytech Univ, Dept Chem Engn Chem & Mat Sci, Metrotech Ctr 6, Brooklyn, NY 11201 USA
关键词
D O I
10.1021/ja021223n
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We report the stability and enzymatic activity of Candida rugosa Lipase (E.C.3.1.1.3) immobilized on γ-Fe2O3 magnetic nanoparticles. The immobilization strategies were either reacting the enzyme amine group with a nanoparticle surface acetyl, or amine groups. In the former, the enzyme was attached through a C=N bond, while in the latter it was connected using glutaraldehyde. AFM images show an average particle size of 20 ± 10 nm after deconvolution. The enzymatic activity of the immobilized lipase was determined by following the ester cleavage of p-nitrophenol butyrate. The covalently immobilized enzyme was stabile and reactive over 30 days. Copyright © 2003 American Chemical Society.
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收藏
页码:1684 / 1685
页数:2
相关论文
共 17 条
[1]   Performance of immobilized glucoamylase in a magnetically stabilized fluidized bed reactor (MSFBR) [J].
Bahar, T ;
Çelebi, SS .
ENZYME AND MICROBIAL TECHNOLOGY, 2000, 26 (01) :28-33
[2]  
CHAPLIN MF, 1990, ENZYME TECHNOLOGY, P13
[4]   Application of magnetic chromatography to the isolation of lysosomes from fibroblasts of patients with lysosomal storage disorders [J].
Diettrich, O ;
Mills, K ;
Johnson, AW ;
Hasilik, A ;
Winchester, BG .
FEBS LETTERS, 1998, 441 (03) :369-372
[5]   High resolution imaging of native biological sample surfaces using scanning probe microscopy [J].
Engel, A ;
Schoenenberger, CA ;
Muller, DJ .
CURRENT OPINION IN STRUCTURAL BIOLOGY, 1997, 7 (02) :279-284
[6]   Polymer synthesis by in vitro enzyme catalysis [J].
Gross, RA ;
Kumar, A ;
Kalra, B .
CHEMICAL REVIEWS, 2001, 101 (07) :2097-2124
[7]   IMMOBILIZATION OF PROTEIN AND POLYSACCHARIDE ON MAGNETIC PARTICLES - SELECTIVE BINDING OF MICROORGANISMS BY CONCANAVALIN A-MAGNETITE [J].
HORISBERGER, M .
BIOTECHNOLOGY AND BIOENGINEERING, 1976, 18 (11) :1647-1651
[8]   Development of magnetic beads for rapid and efficient metal-chelate affinity purifications [J].
Ji, ZS ;
Pinon, DI ;
Miller, LJ .
ANALYTICAL BIOCHEMISTRY, 1996, 240 (02) :197-201
[9]   Recent developments of magnetic beads for use in nucleic acid purification [J].
Levison, PR ;
Badger, SE ;
Dennis, J ;
Hathi, P ;
Davies, MJ ;
Bruce, IJ ;
Schimkat, D .
JOURNAL OF CHROMATOGRAPHY A, 1998, 816 (01) :107-111
[10]   EFFECT OF SURFACE HYDROPHOBICITY ON THE CONFORMATIONAL-CHANGES OF ADSORBED FIBRINOGEN [J].
LU, DR ;
PARK, K .
JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1991, 144 (01) :271-281