Effect of BCG stimulus on proinflammatory cytokine production in laryngeal cancer

被引：10

作者：

Conti-Freitas, Luiz Carlos ^{[1
]}

Foss-Freitas, Maria Cristina ^{[2
]}

Martins Mamede, Rui Celso ^{[1
]}

Foss, Norma Tiraboschi ^{[2
]}

机构：

[1] Univ Sao Paulo, Dept Head & Neck Surg, Ribeirao Preto Med Sch, BR-14049 Ribeirao Preto, Brazil

[2] Univ Sao Paulo, Dept Internal Med, Ribeirao Preto Med Sch, BR-14049 Ribeirao Preto, Brazil

来源：

CANCER IMMUNOLOGY IMMUNOTHERAPY | 2009年 / 58卷 / 01期

关键词：

Cancer; Cytokine; Cell culture; TUMOR-NECROSIS-FACTOR; BACILLUS-CALMETTE-GUERIN; T-CELLS; HEAD; RELEASE; NECK;

D O I：

10.1007/s00262-008-0520-7

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background Evaluate the production of TNF and IL-6 in the supernatant of peripheral blood mononuclear cell (PBMC) cultures of patients with supraglottic laryngeal cancer before and after surgical treatment. Materials and methods Adherent cell cultures were stimulated with LPS and BCG. Fourteen patients with advanced supraglottic laryngeal cancer were studied. Cytokine concentration was determined by ELISA in supernatants of mononuclear cell cultures. Results In non-stimulated cultures, lower TNF cytokine levels were detected during the late postoperative (LP) period compared to control (P = 0.02). LP TNF and IL-6 levels were high in cultures stimulated with LPS compared with the preoperative period (PREOP) (P = 0.007; P = 0.008, respectively). Stimulation with BCG led to increased levels of TNF and IL-6 during the LP period compared to control (P = 0.001; P = 0.04, respectively). Conclusions BCG is able to modulate the immune response of patients with advanced supraglottic laryngeal cancer in vitro, increasing the secretion of TNF and IL-6 by macrophages during the postoperative period.

引用

页码：25 / 29

页数：5

共 23 条

[11] INTERFERON BETA-2/B-CELL STIMULATORY FACTOR TYPE-2 SHARES IDENTITY WITH MONOCYTE-DERIVED HEPATOCYTE-STIMULATING FACTOR AND REGULATES THE MAJOR ACUTE PHASE PROTEIN RESPONSE IN LIVER-CELLS [J].