Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy

被引：1149

作者：

Keller, Philipp J. ^{[1
,2
]}

Schmidt, Annette D. ^{[2
]}

Wittbrodt, Joachim ^{[1
,2
,3
,4
]}

Stelzer, Ernst H. K. ^{[1
]}

机构：

[1] European Mol Biol Lab, Cell Biol & Biophys Unit, D-69117 Heidelberg, Germany

[2] European Mol Biol Lab, Dev Biol Unit, D-69117 Heidelberg, Germany

[3] Heidelberg Univ, Inst Zool, Dept Dev Physiol, INF 230, D-69120 Heidelberg, Germany

[4] KIT, Inst Toxicol & Genet, D-76021 Karlsruhe, Germany

来源：

SCIENCE | 2008年 / 322卷 / 5904期

关键词：

D O I：

10.1126/science.1162493

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A long- standing goal of biology is to map the behavior of all cells during vertebrate embryogenesis. We developed digital scanned laser light sheet fluorescence microscopy and recorded nuclei localization and movement in entire wild- type and mutant zebrafish embryos over the first 24 hours of development. Multiview in vivo imaging at 1.5 billion voxels per minute provides "digital embryos," that is, comprehensive databases of cell positions, divisions, and migratory tracks. Our analysis of global cell division patterns reveals a maternally defined initial morphodynamic symmetry break, which identifies the embryonic body axis. We further derive a model of germ layer formation and show that the mesendoderm forms from one- third of the embryo's cells in a single event. Our digital embryos, with 55 million nucleus entries, are provided as a resource.

引用

页码：1065 / 1069

页数：5

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