Transcription factor Sp1 is essential for early embryonic development but dispensable for cell growth and differentiation

被引：478

作者：

Marin, M ^{[1
]}

Karis, A ^{[1
]}

Visser, P ^{[1
]}

Grosveld, F ^{[1
]}

Philipsen, S ^{[1
]}

机构：

[1] ERASMUS UNIV ROTTERDAM,DEPT CELL BIOL,NL-3000 DR ROTTERDAM,NETHERLANDS

来源：

CELL | 1997年 / 89卷 / 04期

关键词：

D O I：

10.1016/S0092-8674(00)80243-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transcription factor Sp1 has been implicated in the expression of many genes. Moreover, it has been suggested that Sp1 is linked to the maintenance of methylation-free CpG islands, the cell cycle, and the formation of active chromatin structures. We have inactivated the mouse Sp1 gene. Sp1(-/-) embryos are retarded in development, show a broad range of abnormalities, and die around day 11 of gestation. In Sp1(-/-) embryos, the expression of many putative target genes, including cell cycle-regulated genes, is not affected, CpG islands remain methylation free, and active chromatin is formed at the globin loci. However, the expression of the methyl-CpG-binding protein MeCP2 is greatly reduced in Sp1(-/-) embryos. MeCP2 is thought to be required for the maintenance of differentiated cells. We suggest that Sp1 is an important regulator of this process.

引用

页码：619 / 628

页数：10

共 54 条

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