Loop-Mediated Isothermal Amplification for the Event-Specific Detection of Wheat B73-6-1

被引：14

作者：

Cheng, Yang ^{[1
]}

Zhang, Minghui ^{[2
]}

Hu, Kun ^{[1
]}

Sun, Fangda ^{[2
]}

Tao, Ran ^{[3
]}

Gao, Xuejun ^{[2
]}

Luan, Fengxia ^{[1
]}

机构：

[1] Heilongjiang Entry Exit Inspect & Quarantine Bur, Harbin, Peoples R China

[2] Northeast Agr Univ, Res Ctr Life Sci & Biotech, Detecting Ctr Agr Genetically Modified Organism, Harbin, Peoples R China

[3] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100094, Peoples R China

来源：

FOOD ANALYTICAL METHODS | 2014年 / 7卷 / 02期

基金：

中国国家自然科学基金;

关键词：

LAMP; Event-Specific Detection; Wheat B73-6-1; GMO; MYCOBACTERIUM-TUBERCULOSIS COMPLEX; INFECTIOUS-DISEASES; CLINICAL-DIAGNOSIS; RAPID DETECTION; LAMP; DNA; SEQUENCE; ASSAY;

D O I：

10.1007/s12161-013-9718-1

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

To develop effective alternatives for detecting genetically modified organisms, we constructed one loop-mediated isothermal amplification (LAMP) event-specific detection method for wheat B73-6-1 in this study. This event-specific LAMP assay can be performed within 38 min without any PCR equipment and the LAMP products can be directly observed by the naked eye instead of conventional gel electrophoresis analysis. The limits of detection of these established visual LAMP assays were about six copies of haploid wheat-genomic DNA. Furthermore, the high specificity of LAMP assays was determined. High specificity and sensitivity, cost-effectiveness, and low requirement of equipment of the developed event-specific LAMP assay of B73-6-1 allow this method to be useful in transgenic wheat samples analysis, especially in highly processed products.

引用

页码：500 / 505

页数：6

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