Activation of glycoprotein VI (GPVI) and C-type lectin-like receptor-2 (CLEC-2) underlies platelet activation by diesel exhaust particles and other charged/hydrophobic ligands

被引：34

作者：

Alshehri, Osama M. ^{[1
]}

Montague, Samantha ^{[1
]}

Watson, Stephanie ^{[1
]}

Carter, Paul ^{[1
]}

Sarker, Najiat ^{[1
]}

Manne, Bhanu K. ^{[2
]}

Miller, Jeanette L. C. ^{[3
]}

Herr, Andrew B. ^{[3
]}

Pollitt, Alice Y. ^{[1
]}

O'Callaghan, Chris A. ^{[4
]}

Kunapuli, Satya ^{[2
]}

Arman, Monica ^{[1
]}

Hughes, Craig E. ^{[1
]}

Watson, Steve P. ^{[1
]}

机构：

[1] Univ Birmingham, Sch Med, Inst Biomed Res, Ctr Cardiovasc Sci, Birmingham B15 2TT, W Midlands, England

[2] Temple Univ, Sol Sherry Thrombosis Res Ctr, Sch Med, Philadelphia, PA 19140 USA

[3] Cincinnati Childrens Hosp Med Ctr, Div Immunobiol, Cincinnati, OH 45229 USA

[4] Univ Oxford, Nuffield Dept Clin Med, Henry Wellcome Bldg Mol Physiol, Oxford OX3 7BN, England

来源：

BIOCHEMICAL JOURNAL | 2015年 / 468卷

基金：

英国惠康基金;

关键词：

C-type lectin-like receptor-2 (CLEC-2); glycoprotein VI (GPVI); histones; Src; Syk; tyrosine kinase; GAMMA-CHAIN; SIGNALING CASCADE; SYK; COLLAGEN; THROMBOSIS; HISTONES; DIMERIZATION; AGGREGATION; PEPTIDE; AGONIST;

D O I：

10.1042/BJ20150192

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Platelets are activated by a range of stimuli that share little or no resemblance in structure to each other or to recognized ligands, including diesel exhaust particles (DEP), small peptides [4N1-1, Champs (computed helical anti-membrane proteins), LSARLAF (Leu-Ser-Ala-Arg-Leu-Ala-Phe)], proteins (histones) and large polysaccharides (fucoidan, dextran sulfate). This miscellaneous group stimulate aggregation of human and mouse platelets through the glycoproteinVI (GPVI)-FcR gamma-chain complex and/or C-type lectin-like receptor-2 (CLEC-2) as shown using platelets from mice deficient in either or both of these receptors. In addition, all of these ligands stimulate tyrosine phosphorylation in GPVI/CLEC-2-double-deficient platelets, indicating that they bind to additional surface receptors, although only in the case of dextran sulfate does this lead to activation. DEP, fucoidan and dextran sulfate, but not the other agonists, activate GPVI and CLEC-2 in transfected cell lines as shown using a sensitive reporter assay confirming a direct interaction with the two receptors. We conclude that this miscellaneous group of ligands bind to multiple proteins on the cell surface including GPVI and/or CLEC-2, inducing activation. These results have pathophysiological significance in a variety of conditions that involve exposure to activating charged/hydrophobic agents.

引用

页码：459 / 473

页数：15

共 30 条

[21] The Fc receptor gamma-chain and the tyrosine kinase Syk are essential for activation of mouse platelets by collagen [J].

Poole, A ;

Gibbins, JM ;

Turner, M ;

vanVugt, MJ ;

vandeWinkel, JGJ ;

Saito, T ;

Tybulewicz, VLJ ;

Watson, SP .

EMBO JOURNAL, 1997, 16 (09) :2333-2341

[22] Extracellular histones promote thrombin generation through platelet-dependent mechanisms: involvement of platelet TLR2 and TLR4 [J].

Semeraro, Fabrizio ;

Ammollo, Concetta T. ;

Morrissey, James H. ;

Dale, George L. ;

Friese, Paul ;

Esmon, Naomi L. ;

Esmon, Charles T. .

BLOOD, 2011, 118 (07) :1952-1961

[23] The tyrosine phosphatase CD148 is an essential positive regulator of platelet activation and thrombosis [J].

Senis, Yotis A. ;

Tomlinson, Michael G. ;

Ellison, Stuart ;

Mazharian, Alexandra ;

Lim, Jenson ;

Zhao, Yan ;

Kornerup, Kristin N. ;

Auger, Jocelyn M. ;

Thomas, Steve G. ;

Dhanjal, Tarvinder ;

Kalia, Neena ;

Zhu, Jing W. ;

Weiss, Arthur ;

Watson, Steve P. .

BLOOD, 2009, 113 (20) :4942-4954

[24] Syk-dependent Phosphorylation of CLEC-2 A NOVEL MECHANISM OF HEM-IMMUNORECEPTOR TYROSINE-BASED ACTIVATION MOTIF SIGNALING [J].

Severin, Sonia ;

Pollitt, Alice Y. ;

Navarro-Nunez, Leyre ;

Nash, Craig A. ;

Mourao-Sa, Diego ;

Eble, Johannes A. ;

Senis, Yotis A. ;

Watson, Steve P. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2011, 286 (06) :4107-4116

[25] Induction of platelet aggregation after a direct physical interaction with diesel exhaust particles [J].

Solomon, A. ;

Smyth, E. ;

Mitha, N. ;

Pitchford, S. ;

Vydyanath, A. ;

Luther, P. K. ;

Thorley, A. J. ;

Tetley, T. D. ;

Emerson, M. .

JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2013, 11 (02) :325-334

[26] The novel Syk inhibitor R406 reveals mechanistic differences in the initiation of GPVI and CLEC-2 signaling in platelets [J].

Spalton, J. C. ;

Mori, J. ;

Pollitt, A. Y. ;

Hughes, C. E. ;

Eble, J. A. ;

Watson, S. P. .

JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2009, 7 (07) :1192-1199

[27] Collagen promotes sustained glycoprotein VI signaling in platelets and cell lines [J].

Tomlinson, M. G. ;

Calaminus, S. D. ;

Berlanga, O. ;

Auger, J. M. ;

Bori-Sanz, T. ;

Meyaard, L. ;

Watson, S. P. .

JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2007, 5 (11) :2274-2283

[28] C-terminal peptide of thrombospondin-1 induces platelet aggregation through the Fc receptor γ-chain-associated signaling pathway and by agglutination [J].

Tulasne, D ;

Judd, BA ;

Johanson, M ;

Asazuma, N ;

Best, D ;

Brown, EJ ;

Kahn, M ;

Koretzky, GA ;

Watson, SP .

BLOOD, 2001, 98 (12) :3346-3352

[29] GPVI and CLEC-2 in hemostasis and vascular integrity [J].

Watson, S. P. ;

Herbert, J. M. J. ;

Pollitt, A. Y. .

JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2010, 8 (07) :1457-1467

[30] NETosis: how vital is it? [J].

Yipp, Bryan G. ;

Kubes, Paul .

BLOOD, 2013, 122 (16) :2784-2794

← 1 2 3 →