Characterization, cloning, and evolutionary history of the chloroplast and cytosolic class I aldolases of the red alga Galdieria sulphuraria

被引:54
作者
Gross, W
Lenze, D
Nowitzki, U
Weiske, J
Schnarrenberger, C
机构
[1] Free Univ Berlin, Inst Pflanzenphysiol & Mikrobiol, D-14195 Berlin, Germany
[2] Tech Univ Carolo Wilhelmina Braunschweig, Inst Genet, D-38023 Braunschweig, Germany
关键词
chloroplastic and cytosolic isoenzymes; evolutionary tree analysis; fructose-1,6-bisphosphate aldolase;
D O I
10.1016/S0378-1119(99)00059-1
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Two fructose-1,6-bisphosphate aldolases from the acido- and thermophilic red alga Galdieria sulphuraria were purified to apparent homogeneity and N-terminally microsequenced. Both aldolases had similar biochemical properties such as Km (FBP) (5.6-5.8 mu M) and molecular masses of the native enzymes (165 kDa) as determined by size exclusion chromatography. The subunit size of the purified aldolases, as determined by SDS-PAGE, was 42 kDa for both aldolases. The isoenzymes were not inhibited by EDTA or affected by cysteine or potassium ions, implying that they belong to the class I group of aldolases, while other red algae are known to have one class I and one class II aldolase inhibited by EDTA. cDNA clones of the cytosolic and plastidic aldolases were isolated and sequenced. The gene for the cytosolic isoenzyme contained a 303 bp untranslated leader sequence, while the gene for the plastidic isoenzyme exhibited a transit sequence of 56 amino-acid residues. Both isoenzymes showed about 48% homology in the deduced amino-acid sequences. A gene tree relates both aldolases to the basis of early eukaryotic class I aldolases. The phylogenetic relationship to other aldolases, particularly to cyanobacterial class II aldolases, is discussed. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:7 / 14
页数:8
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