Fission Yeast Scm3: A CENP-A Receptor Required for Integrity of Subkinetochore Chromatin

被引：157

作者：

Pidoux, Alison L. ^{[1
,2
]}

Choi, Eun Shik ^{[1
,2
]}

Abbott, Johanna K. R. ^{[1
,2
]}

Liu, Xingkun ^{[3
]}

Kagansky, Alexander ^{[1
,2
]}

Castillo, Araceli G. ^{[1
,2
]}

Hamilton, Georgina L. ^{[1
,2
]}

Richardson, William ^{[4
]}

Rappsilber, Juri ^{[1
,2
]}

He, Xiangwei ^{[3
]}

Allshire, Robin C. ^{[1
,2
]}

机构：

[1] Univ Edinburgh, Wellcome Trust Ctr Cell Biol, Edinburgh EH9 3JR, Midlothian, Scotland

[2] Univ Edinburgh, Inst Cell Biol, Sch Biol Sci, Edinburgh EH9 3JR, Midlothian, Scotland

[3] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA

[4] MRC Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland

来源：

MOLECULAR CELL | 2009年 / 33卷 / 03期

基金：

英国惠康基金;

关键词：

HISTONE FOLD DOMAIN; SPINDLE POLE BODY; CENTROMERIC CHROMATIN; CHROMOSOME SEGREGATION; CELL CYCLE; PROTEIN; KINETOCHORE; COMPLEX; PROTEOMICS; H3;

D O I：

10.1016/j.molcel.2009.01.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mechanisms ensuring specific incorporation of CENP-A at centromeres are poorly understood. Mis16 and Mis18 are required for CENP-A localization at centromeres and form a complex that is conserved from fission yeast to human. Fission yeast sim1 mutants that alleviate kinetochore domain silencing are defective in Scm3(Sp), the ortholog of budding yeast Scm3(Sc). Scm3(Sp) depends on Mis16/18 for its centromere localization and like them is recruited to centromeres in late anaphase. Importantly, Scm3(Sp) coaffinity purifies with CENP-A(Cnp1) and associates with CENP-A(Cnp1) in vitro, yet localizes independently of intact CENP-A(Cnp1) chromatin and is differentially released from chromatin. While Scm3(Sc) has been proposed to form a unique hexameric nucleosome with CENP-A(Cse4) and histone H4 at budding yeast point centromeres, we favor a model in which Scm3(Sp) acts as a CENP-A(Cnp1) receptor/assembly factor, cooperating with Mis16 and Mis18 to receive CENP-A(Cnp1) from the Sim3 escort and mediate assembly of CENP-A(Cnp1) into subkinetochore chromatin.

引用

页码：299 / 311

页数：13

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