Cell cycle-regulated recognition of the destruction box of cyclin B by the APC/C in Xenopus egg extracts

被引：107

作者：

Yamano, H ^{[1
]}

Gannon, J ^{[1
]}

Mahbubani, H ^{[1
]}

Hunt, T ^{[1
]}

机构：

[1] Canc Res UK, Clare Hall Labs, S Mimms EN6 3LD, Herts, England

来源：

MOLECULAR CELL | 2004年 / 13卷 / 01期

基金：

日本科学技术振兴机构;

关键词：

D O I：

10.1016/S1097-2765(03)00480-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Substrates for mitotic proteolysis such as cyclin B have a 9 residue destruction motif, the destruction box (D-box). To identify the receptor that specifically binds the D-box, we used affinity chromatography with immobilized D-box matrices. We find that the APC/C from Xenopus egg extracts binds to the D-box of cyclin B, whereas Fizzy (Cdc20) does not. Mutations in the D-box abolished this interaction. We show that this binding is regulated in the cell cycle, such that the APC/C from egg extracts in interphase does not bind to the D-box matrix. Our results suggest that the APC/C forms a stable interaction with the D-box of its substrates in a cell cycle-dependent manner.

引用

页码：137 / 147

页数：11

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