Mapping and identification of essential gene functions on the X chromosome of Drosophila

被引:97
作者
Peter, A
Schöttler, P
Werner, M
Beinert, N
Dowe, G
Burkert, P
Mourkioti, F
Dentzer, L
He, YC
Deak, P
Benos, PV
Gatt, MK
Murphy, L
Harris, D
Barrell, B
Ferraz, C
Vidal, S
Brun, C
Demaille, J
Cadieu, E
Dreano, S
Gloux, S
Lelaure, V
Mottier, S
Galibert, F
Borkova, D
Miñana, B
Kafatos, FC
Bolshakov, S
Sidén-Kiamos, I
Papagiannakis, G
Spanos, L
Louis, C
Madueño, E
de Pablos, B
Modolell, J
Bucheton, A
Callister, D
Campbell, L
Henderson, NS
McMillan, PJ
Salles, C
Tait, E
Valenti, P
Saunders, RDC
Billaud, A
Pachter, L
Klapper, R
Janning, W
Glover, DM
机构
[1] Max Planck Inst Biophys Chem, Abt Mol Entwicklungsbiol, D-37077 Gottingen, Germany
[2] European Mol Biol Lab, D-69012 Heidelberg, Germany
[3] Univ Munster, Inst Allgemeine Zool & Genet, D-48149 Munster, Germany
[4] Baylor Coll Med, Howard Hughes Med Inst, Dept Mol & Human Genet, Houston, TX 77030 USA
[5] Univ Calif Berkeley, Dept Math, Berkeley, CA 94720 USA
[6] Univ Cambridge, Dept Genet, Cambridge CB2 3EH, England
[7] European Bioinformat Inst, EMBL Outstn, Cambridge CB10 1SD, England
[8] Sanger Ctr, Cambridge CB10 1SA, England
[9] Univ Dundee, Dept Anat & Physiol, CRC Cell Cycle Genet Grp, Dundee DD1 4HN, Scotland
[10] Open Univ, Dept Biol Sci, Milton Keynes MK7 6AA, Bucks, England
[11] Univ Montpellier, Sch Med, CNRS, Inst Genet Humaine, F-34396 Montpellier 5, France
[12] Ctr Etud Polymorphisme Humain, Fdn Jean Dausset, F-75010 Paris, France
[13] FORTH, Inst Mol Biol & Biotechnol, Iraklion, Greece
[14] Univ Crete, Dept Biol, Iraklion, Greece
[15] Univ Autonoma Madrid, E-28049 Madrid, Spain
[16] CSIC, Ctr Biol Mol Severo Ochoa, E-28049 Madrid, Spain
关键词
D O I
10.1093/embo-reports/kvf012
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Drosophila melanogaster genome consists of four chromosomes that contain 165 Mb of DNA, 120 Mb of which are euchromatic. The two Drosophila Genome Projects, in collaboration with Celera Genomics Systems, have sequenced the genome, complementing the previously established physical and genetic maps. In addition, the Berkeley Drosophila Genome Project has undertaken large-scale functional analysis based on mutagenesis by transposable P element insertions into autosomes. Here, we present a large-scale P element insertion screen for vital gene functions and a BAC tiling map for the X chromosome. A collection of 501 X-chromosomal P element insertion lines was used to map essential genes cytogenetically and to establish short sequence tags (STSs) linking the insertion sites to the genome. The distribution of the P element integration sites, the identified genes and transcription units as well as the expression patterns of the P-element-tagged enhancers is described and discussed.
引用
收藏
页码:34 / 38
页数:5
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