From sequence to chromosome:: The tip of the X chromosome of D. melanogaster

被引:22
作者
Benos, PV
Gatt, MK
Ashburner, M
Murphy, L
Harris, D
Barrell, B
Ferraz, C
Vidal, S
Brun, C
Demailles, J
Cadieu, E
Dreano, S
Gloux, S
Lelaure, V
Mottier, S
Galibert, F
Borkova, D
Minana, B
Kafatos, FC
Louis, C
Sidén-Kiamos, I
Bolshakov, S
Papagiannakis, G
Spanos, L
Cox, S
Madueño, E
de Pablos, B
Modolell, J
Peter, A
Schöttler, P
Werner, M
Mourkioti, F
Beinert, N
Dowe, G
Schäfer, U
Jäckle, H
Bucheton, A
Callister, DM
Campbell, LA
Darlamitsou, A
Henderson, NS
McMillan, PJ
Salles, C
Tait, EA
Valenti, P
Saunders, RDC
Glover, DM
机构
[1] European Bioinformat Inst, Cambridge CB10 1SD, England
[2] Univ Cambridge, Dept Genet, Cambridge CB2 3EH, England
[3] Sanger Ctr, Cambridge CB10 1SA, England
[4] Univ Montpellier, Sch Med, IGH, CNRS, F-34396 Montpellier 5, France
[5] Fac Med, CNRS, Unite Propre Rech 41, F-35043 Rennes, France
[6] European Mol Biol Lab, Heidelberg, Germany
[7] Fdn Res & Technol Hellas, Inst Mol Biol & Biotechnol, Heraklion, Greece
[8] Univ Crete, Dept Biol, Heraklion, Greece
[9] Univ Autonoma Madrid, E-28049 Madrid, Spain
[10] CSIC, Ctr Biol Mol Severo Ochoa, E-28049 Madrid, Spain
[11] Max Planck Inst Biophys Chem, Dept Mol Dev Biol, D-37070 Gottingen, Germany
[12] Univ Dundee, Dept Anat & Physiol, Canc Res Campaign, Cell Cycle Genet Grp, Dundee DD1 4HN, Scotland
[13] Open Univ, Dept Biol Sci, Milton Keynes MK7 6AA, Bucks, England
关键词
D O I
10.1126/science.287.5461.2220
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
One of the rewards of having a Drosophila melanogaster whole-genome sequence will be the potential to understand the molecular bases for structural features of chromosomes that have been a Long-standing puzzle. Analysis of 2.6 megabases of sequence from the tip of the X chromosome of Drosophila identifies 273 genes. Cloned DNAs from the characteristic bulbous structure at the tip of the X chromosome in the region of the broad complex display an unusual pattern of in situ hybridization. Sequence analysis revealed that this region comprises 154 kilobases of DNA flanked by 1.2-kilobases of inverted repeats, each composed of a 350-base pair satellite related element. Thus, some aspects of chromosome structure appear to be revealed directly within the DNA sequence itself.
引用
收藏
页码:2220 / 2222
页数:3
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