Trichoderma-Plant Root Colonization: Escaping Early Plant Defense Responses and Activation of the Antioxidant Machinery for Saline Stress Tolerance

被引:262
作者
Brotman, Yariv [1 ]
Landau, Udi [2 ]
Cuadros-Inostroza, Alvaro [1 ]
Takayuki, Tohge [1 ]
Fernie, Alisdair R. [1 ]
Chet, Ilan [2 ]
Viterbo, Ada [2 ]
Willmitzer, Lothar [1 ]
机构
[1] Max Planck Inst Mol Plant Physiol, Potsdam, Germany
[2] Hebrew Univ Jerusalem, Dept Plant Pathol & Microbiol, Robert H Smith Fac Agr Food & Environm, IL-76100 Rehovot, Israel
关键词
INDUCED SYSTEMIC RESISTANCE; ARABIDOPSIS TRANSCRIPTION FACTOR; FUNGUS PIRIFORMOSPORA-INDICA; SALICYLIC-ACID; DISEASE-RESISTANCE; ACC-DEAMINASE; MONOOXYGENASE FMO1; BOTRYTIS-CINEREA; SALT TOLERANCE; GENE;
D O I
10.1371/journal.ppat.1003221
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Trichoderma spp. are versatile opportunistic plant symbionts which can colonize the apoplast of plant roots. Microarrays analysis of Arabidopsis thaliana roots inoculated with Trichoderma asperelloides T203, coupled with qPCR analysis of 137 stress responsive genes and transcription factors, revealed wide gene transcript reprogramming, proceeded by a transient repression of the plant immune responses supposedly to allow root colonization. Enhancement in the expression of WRKY18 and WRKY40, which stimulate JA-signaling via suppression of JAZ repressors and negatively regulate the expression of the defense genes FMO1, PAD3 and CYP71A13, was detected in Arabidopsis roots upon Trichoderma colonization. Reduced root colonization was observed in the wrky18/wrky40 double mutant line, while partial phenotypic complementation was achieved by over-expressing WRKY40 in the wrky18 wrky40 background. On the other hand increased colonization rate was found in roots of the FMO1 knockout mutant. Trichoderma spp. stimulate plant growth and resistance to a wide range of adverse environmental conditions. Arabidopsis and cucumber (Cucumis sativus L.) plants treated with Trichoderma prior to salt stress imposition show significantly improved seed germination. In addition, Trichoderma treatment affects the expression of several genes related to osmo-protection and general oxidative stress in roots of both plants. The MDAR gene coding for monodehydroascorbate reductase is significantly up-regulated and, accordingly, the pool of reduced ascorbic acid was found to be increased in Trichoderma treated plants. 1-Aminocyclopropane-1-carboxylate (ACC)-deaminase silenced Trichoderma mutants were less effective in providing tolerance to salt stress, suggesting that Trichoderma, similarly to ACC deaminase producing bacteria, can ameliorate plant growth under conditions of abiotic stress, by lowering ameliorating increases in ethylene levels as well as promoting an elevated antioxidative capacity.
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页数:15
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共 77 条
[1]   Systemic modulation of gene expression in tomato by Trichoderma hamatum 382 [J].
Alfano, G. ;
Ivey, M. L. Lewis ;
Cakir, C. ;
Bos, J. I. B. ;
Miller, S. A. ;
Madden, L. V. ;
Kamoun, S. ;
Hoitink, H. A. J. .
PHYTOPATHOLOGY, 2007, 97 (04) :429-437
[2]   Trichoderma virens, a Plant Beneficial Fungus, Enhances Biomass Production and Promotes Lateral Root Growth through an Auxin-Dependent Mechanism in Arabidopsis [J].
Angel Contreras-Cornejo, Hexon ;
Macias-Rodriguez, Lourdes ;
Cortes-Penagos, Carlos ;
Lopez-Bucio, Jose .
PLANT PHYSIOLOGY, 2009, 149 (03) :1579-1592
[3]   Colonization of Arabidopsis roots by Trichoderma atroviride promotes growth and enhances systemic disease resistance through jasmonic acid/ethylene and salicylic acid pathways [J].
Angel Salas-Marina, Miguel ;
Angel Silva-Flores, Miguel ;
Elena Uresti-Rivera, Edith ;
Castro-Longoria, Ernestina ;
Herrera-Estrella, Alfredo ;
Casas-Flores, Sergio .
EUROPEAN JOURNAL OF PLANT PATHOLOGY, 2011, 131 (01) :15-26
[4]  
Bae H, 2011, MOL PLANT MICROBE IN, V24, P628
[5]   Salicylic acid-independent ENHANCED DISEASE SUSCEPTIBILITY1 signaling in Arabidopsis immunity and cell death is regulated by the monooxygenase FMO1 and the nudix hydrolase NUDT7 [J].
Bartsch, M ;
Gobbato, E ;
Bednarek, P ;
Debey, S ;
Schultze, JL ;
Bautor, J ;
Parker, JE .
PLANT CELL, 2006, 18 (04) :1038-1051
[6]   PLANT PRODUCTIVITY AND ENVIRONMENT [J].
BOYER, JS .
SCIENCE, 1982, 218 (4571) :443-448
[7]   Role of swollenin, an expansin-like protein from Trichoderma, in plant root colonization [J].
Brotman, Yariv ;
Briff, Eden ;
Viterbo, Ada ;
Chet, Ilan .
PLANT PHYSIOLOGY, 2008, 147 (02) :779-789
[8]   Transcript and metabolite analysis of the Trichoderma-induced systemic resistance response to Pseudomonas syringae in Arabidopsis thaliana [J].
Brotman, Yariv ;
Lisec, Jan ;
Meret, Michael ;
Chet, Ilan ;
Willmitzer, Lothar ;
Viterbo, Ada .
MICROBIOLOGY-SGM, 2012, 158 :139-146
[9]   Trichoderma [J].
Brotman, Yariv ;
Kapuganti, J. Gupta ;
Viterbo, Ada .
CURRENT BIOLOGY, 2010, 20 (09) :R390-R391
[10]   Ethylene signalling and ethylene-targeted transcription factors are required to balance beneficial and nonbeneficial traits in the symbiosis between the endophytic fungus Piriformospora indica and Arabidopsis thaliana [J].
Camehl, Iris ;
Sherameti, Irena ;
Venus, Yvonne ;
Bethke, Gerit ;
Varma, Ajit ;
Lee, Justin ;
Oelmueller, Ralf .
NEW PHYTOLOGIST, 2010, 185 (04) :1062-1073