Characterization of a functional neuropeptide F receptor from Drosophila melanogaster

被引：130

作者：

Garczynski, SF

Brown, MR

Shen, P

Murray, TF

Crim, JW ^{[1
]}

机构：

[1] Univ Georgia, Dept Cellular Biol, Athens, GA 30602 USA

[2] Univ Georgia, Dept Entomol, Athens, GA 30602 USA

[3] Univ Georgia, Dept Physiol & Pharmacol, Athens, GA 30602 USA

来源：

PEPTIDES | 2002年 / 23卷 / 04期

关键词：

insect; diptera; Drosophila; G protein-coupled receptor; neuropeptide F; neuropeptide Y;

D O I：

10.1016/S0196-9781(01)00647-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Potential receptors for Drosophila neuropeptide F (DmNPF) were identified in the genome database. One receptor (DmNPFR I) sequence resembled the Lymnaea NPY receptor, an invertebrate homolog of the vertebrate Y-receptor family. DmNPFR I was cloned and tested for functionality in stably transfected mammalian CHO cells. In whole cell binding assays, DmNPF displaced I-125-NPF in a concentration-dependent manner (IC50 = 65 nM). DmNPF inhibited forskolin-stimulated adenylyl cyclase activity similarly (IC50 = 51 nM). Wholemount in situ hybridization revealed that DmNPFR1 RNA is expressed in CNS and midgut of Drosophila larvae. DmNPFR1, a new invertebrate Y-receptor homolog, apparently is a functional receptor for DmNPF. (C) 2002 Elsevier Science Inc. All rights reserved.

引用

页码：773 / 780

页数：8

共 20 条

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