Tumour necrosis factor-alpha up-regulates decay-accelerating factor gene expression in human intestinal epithelial cells

被引：37

作者：

Andoh, A ^{[1
]}

Fujiyama, Y ^{[1
]}

Sumiyoshi, K ^{[1
]}

Sakumoto, H ^{[1
]}

Okabe, H ^{[1
]}

Bamba, T ^{[1
]}

机构：

[1] SHIGA UNIV MED SCI,DEPT CLIN LAB MED,OTSU,SHIGA 52021,JAPAN

来源：

IMMUNOLOGY | 1997年 / 90卷 / 03期

关键词：

D O I：

10.1111/j.1365-2567.1997.00358.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The increased expression of decay-accelerating factor (DAF) has been detected in intestinal epithelial cells at the inflamed mucosa. In this study, we examined the effects of tumour necrosis factor (TNF)-alpha on DAF expression in three intestinal epithelial cell lines. DAF mRNA expression was evaluated by Northern blot analysis, and DAF protein expression was analysed by biotin labelling and immunoprecipitation TNF-alpha induced a marked increase in DAF mRNA and protein expression in HT-29, T84 and Caco-2 cells. In HT-29 cells, the effects of TNF-a on DAF mRNA accumulation were observed in a dose-dependent manner; DAF mRNA accumulation reached a maximum at 3-6 hr, and then gradually decreased. These effects of TNF-alpha required de novo protein synthesis. Messenger RNA stability studies suggested that TNF-alpha partially regulated DAF gene expression by a posttranscriptional mechanism. Moreover, the combination of TNF-alpha and interleukin (IL)-4 induced an additive increase in DAF mRNA accumulation in HT-29 and T84 cells. In human intestinal epithelial cells, TNF-alpha acts as a potent inducer of DAF mRNA expression, indicating an important role for TNF-alpha in the regulation of DAF expression at the inflamed mucosa.

引用

页码：358 / 363

页数：6

共 35 条

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