A Pyrrolysine Analogue for Protein Click Chemistry

被引：96

作者：

Fekner, Tomasz

Li, Xin

Lee, Marianne M.

Chan, Michael K. ^{[1
]}

机构：

[1] Ohio State Univ, Dept Chem, Columbus, OH 43210 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 09期

基金：

美国国家卫生研究院;

关键词：

amino acids; calmodulin; click chemistry; FRET; pyrrolysine; TRANSFER-RNA SYNTHETASES; GENETIC-CODE; ESCHERICHIA-COLI; BACTERIAL-CELLS; CYCLOADDITION; ALDEHYDES; EFFICIENT; RESIDUE; ACIDS; UAG;

D O I：

10.1002/anie.200805420

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) Ignoring the STOP sign: A pyrrolysine analogue bearing a terminal alkyne was site-specifically incorporated into recombinant calmodulin (CaM) through a UAG codon. The resulting protein was labeled with an azide-containing dye using a copper(I)-catalyzed click reaction. Subsequent application of an orthogonal cysteine tagging method yielded a CaM labeled with two distinct fluorophores that enabled its study by FRET spectroscopy. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

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页码：1633 / 1635

页数：3

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