Producing nature's gene-chips: The generation of peptides for display by MHC class I molecules

被引:237
作者
Shastri, N [1 ]
Schwab, S [1 ]
Serwold, T [1 ]
机构
[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Div Immunol, Berkeley, CA 94720 USA
关键词
antigen processing; antigen presentation; proteasome; ER proteolysis;
D O I
10.1146/annurev.immunol.20.100301.064819
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Gene-chips contain thousands of nucleotide sequences that allow simultaneous analysis of the complex mixture of RNAs transcribed in cells. Like these gene-chips, major histocompatibility complex (MHC) class I molecules display a large array of peptides on the cell surface for probing by the CD8(+) T cell repertoire. The peptide mixture represents fragments of most, if not an, intracellular proteins. The antigen processing machinery accomplishes the daunting task of sampling these proteins and cleaving them into the precise set of peptides displayed by MHC I molecules. It has long been believed that antigenic peptides arose as by-products of normal protein turnover. Recent evidence, however, suggests that the primary source of peptides is newly synthesized proteins that arise from conventional as well as cryptic translational reading frames. It is increasingly clear that for many peptides the C-terminus is generated in the cytoplasm, and N-terminal trimming occurs in the endoplasmic reticulum in an MEC I-dependent manner. Nature's gene-chips are thus both parsimonious and elegant.
引用
收藏
页码:463 / 493
页数:31
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