Analysis of activated GAPs and GEFs in cell lysates

被引：161

作者：

García-Mata, R

Wennerberg, K

Arthur, WT

Noren, NK

Ellerbroek, SM

Burridge, K

机构：

[1] Cytoskeleton, Denver, CO USA

[2] Burnham Inst, La Jolla, CA 92037 USA

[3] Warburg Coll, Dept Chem, Waverly, IA USA

[4] Univ N Carolina, Dept Cell & Dev Biol, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USA

来源：

METHODS IN ENZYMOLOGY, VOL 406, REGULATORS AND EFFECTORS OF SMALL GTPASES: RHO FAMILY | 2006年 / 406卷

关键词：

D O I：

10.1016/S0076-6879(06)06031-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An assay was developed that allows the precipitation of the active pools of Rho-GEFs, Rho-GAPs, or effectors from cell or tissue lysates. This assay can be used to identify GEFs, GAPS, and effectors involved in specific cellular pathways to determine their GTPase specificity and to monitor the temporal activation of GEFs and GAPS in response to upstream signals.

引用

收藏

页码：425 / 437

页数：13

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