Separation, purification, and α-glucosidase inhibition of polysaccharides from Coriolus versicolor LH1 mycelia

被引：109

作者：

Hsu, Wen-kuang ^{[1
]}

Hsu, Tai-hao ^{[2
,3
]}

Lin, Fang-yi ^{[2
]}

Cheng, Yuan-kai ^{[1
]}

Yang, John Po-wen ^{[1
]}

机构：

[1] Dayeh Univ, Dept Bioind Technol, Nat Prod Chem Res Lab, Changhua 51591, Taiwan

[2] Dayeh Univ, Dept Bioind Technol, Microbiol Lab, Changhua 51591, Taiwan

[3] Da Yeh Univ, Dept Med Bot & Healthcare, Microbiol Lab, Changhua 51591, Taiwan

来源：

CARBOHYDRATE POLYMERS | 2013年 / 92卷 / 01期

关键词：

Coriolus versicolor LH1; Intracellular polysaccharides; alpha-Glucosidase inhibitory properties; Diaion (R) HP20 macroporous adsorption; SUBMERGED FERMENTATION CULTURE; EXTRACELLULAR POLYSACCHAROPEPTIDES; PEPTIDE PSP; CYCLOPHOSPHAMIDE; QUANTIFICATION; IDENTIFICATION; FRACTIONATION; PERFORMANCE; CELL;

D O I：

10.1016/j.carbpol.2012.10.001

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Intracellular polysaccharides (iPs) were separated and purified from Coriolus versicolor LH1 mycelia and characterized for their alpha-glucosidase inhibitory properties. Three iP fractions (iPL-F5-2-1, iPL-F5-4-1, and iPL-F5-5-1) were extracted, separated, and purified from LH1 mycelia using microwave extraction technology, a DEAE-Sepharose (TM) CL-6B column, a Diaion (R) HP20 macroporous adsorption column, and a Sephadex (TM) G-50 gel-permeation column. The principal constituents of iPL-F5-2-1, iPL-F5-4-1 and iPL-F5-5-1 were saponins and polyphenoic compound mixtures. The enzyme inhibition activity. IC50 values, of these three fractions were 1.7, 1.8, and 0.8 mg/mL, respectively. The alpha-glucosidase inhibitory properties were related to the presence of alpha-(1,4)glycosidic linkages in the polysaccharide structure and the total relative percentage of D-glucose and D-galactose in the structure of polysaccharides, other than triterpenoids. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：297 / 306

页数：10

共 38 条

[1]

Effects of polysaccharide peptide (PSP) from Coriolus versicolor on the pharmacokinetics of cyclophosphamide in the rat and cytotoxicity in HepG2 cells [J].