Increased thermostability of three mesophilic beta-galactosidases under high pressure

被引：24

作者：

Athes, V ^{[1
]}

Degraeve, P ^{[1
]}

CavailleLefebvre, D ^{[1
]}

Espeillac, S ^{[1
]}

Lemay, P ^{[1
]}

Combes, D ^{[1
]}

机构：

[1] INST NATL SCI APPL,CTR BIOINGN GILBERT DURAND,CNRS UMR5504,LA INRA,F-31077 TOULOUSE 4,FRANCE

来源：

BIOTECHNOLOGY LETTERS | 1997年 / 19卷 / 03期

关键词：

D O I：

10.1023/A:1018317926631

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

High pressure (>200 Mpa) or high temperature (>45 degrees C) can both induce an irreversible inactivation of the beta-galactosidases of Aspergillus oryzae, Kluyveromyces lactis and Escherichia coli. Moderate pressures (50 MPa-250 MPa) exerted a protective effect against thermal inactivation for the three beta-galactosidases investigated. High pressure could thus be used to carry out beta-galactosidase catalysed reactions such as lactose hydrolysis, at higher temperatures.

引用

页码：273 / 276

页数：4

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