Architecture of the SWI/SNF-nucleosome complex

被引：108

作者：

Dechassa, Mekonnen Lemma ^{[1
]}

Zhang, Bei ^{[1
,4
]}

Horowitz-Scherer, Rachel ^{[2
]}

Persinger, Jim ^{[1
]}

Woodcock, Christopher L. ^{[2
]}

Peterson, Craig L. ^{[3
]}

Bartholomew, Blaine ^{[1
]}

机构：

[1] So Illinois Univ, Dept Biochem & Mol Biol, Carbondale, IL 62901 USA

[2] Univ Massachusetts, Dept Biol, Amherst, MA 01003 USA

[3] Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA 01605 USA

[4] Thomas Univ, Dept Biol, Thomasville, GA 31792 USA

来源：

MOLECULAR AND CELLULAR BIOLOGY | 2008年 / 28卷 / 19期

关键词：

D O I：

10.1128/MCB.00693-08

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The SWI/SNF complex disrupts and mobilizes chromatin in an ATP-dependent manner. SWI/SNF interactions with nucleosomes were mapped by DNA footprinting and site-directed DNA and protein cross-linking when SWI/SNF was recruited by a transcription activator. SWI/SNF was found by DNA footprinting to contact tightly around one gyre of DNA spanning similar to 50 bp from the nucleosomal entry site to near the dyad axis. The DNA footprint is consistent with nucleosomes binding to an asymmetric trough of SWI/SNF that was revealed by the improved imaging of free SWI/SNF. The DNA site-directed cross-linking revealed that the catalytic subunit Swi2/Snf2 is associated with nucleosomes two helical turns from the dyad axis and that the Snf6 subunit is proximal to the transcription factor recruiting SWI/SNF. The highly conserved Snf5 subunit associates with the histone octamer and not with nucleosomal DNA. The model of the binding trough of SWI/SNF illustrates how nucleosomal DNA can be mobilized while SWI/SNF remains bound.

引用

页码：6010 / 6021

页数：12

共 54 条

[31] Transcription activator interactions with multiple SWI/SNF subunits [J].

Neely, KE ;

Hassan, AH ;

Brown, CE ;

Howe, L ;

Workman, JL .

MOLECULAR AND CELLULAR BIOLOGY, 2002, 22 (06) :1615-1625

[32] Persistent site-specific remodeling of a nucleosome array by transient action of the SWI/SNF complex [J].

OwenHughes, T ;

Utley, RT ;

Cote, J ;

Peterson, CL ;

Workman, JL .

SCIENCE, 1996, 273 (5274) :513-516

[33] Mapping the contacts of yeast TFIIIB and RNA polymerase III at various distances from the major groove of DNA by DNA photoaffinity labeling [J].

Persinger, J ;

Bartholomew, B .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (51) :33039-33046

[34] Cellular machineries for chromosomal DNA repair [J].

Peterson, CL ;

Côté, J .

GENES & DEVELOPMENT, 2004, 18 (06) :602-616

[35] Octamer transfer and creation of stably remodeled nucleosomes by human SWI-SNF and its isolated ATPases [J].

Phelan, ML ;

Schnitzler, GR ;

Kingston, RE .

MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (17) :6380-6389

[36] Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits [J].

Phelan, ML ;

Sif, S ;

Narlikar, GJ ;

Kingston, RE .

MOLECULAR CELL, 1999, 3 (02) :247-253

[37] Chromatin remodeling through directional DNA translocation from an internal nucleosomal site [J].

Saha, A ;

Wittmeyer, J ;

Cairns, BR .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2005, 12 (09) :747-755

[38] Use of DNA photoaffinity labeling to study nucleosome remodeling by SWI/SNF [J].

Sengupta, SM ;

Persinger, J ;

Bartholomew, B ;

Peterson, CL .

METHODS-A COMPANION TO METHODS IN ENZYMOLOGY, 1999, 19 (03) :434-446

[39] The interactions of yeast SWI/SNF and RSC with the nucleosome before and after chromatin remodeling [J].

Sengupta, SM ;

VanKanegan, M ;

Persinger, J ;

Logie, C ;

Cairns, BR ;

Peterson, CL ;

Bartholomew, B .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (16) :12636-12644

[40]

Shen F, 1996, J BIOL CHEM, V271, P4819

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