Retention mechanism of analytes in the solid-phase extraction process using molecularly imprinted polymers - Application to the extraction of triazines from complex matrices

被引:108
作者
Chapuis, F
Pichon, V
Lanza, F
Sellergren, B
Hennion, MC
机构
[1] CNRS, UMR 7121, Ecole Super Phys & Chim Ind, Dept Environm & Analit Chem, F-75231 Paris 05, France
[2] Univ Dortmund, INFU, D-44221 Dortmund, Germany
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2004年 / 804卷 / 01期
关键词
retention mechanism; molecularly imprinted polymers; triazines;
D O I
10.1016/j.jchromb.2003.12.033
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Two molecularly imprinted polymers (MIPs), prepared in dichloromethane with terbutylazine and ametryn as template molecules, were evaluated for the selective extraction of triazines from complex matrices. Various parameters affecting extraction recoveries on MIPs were studied in order to obtain an optimized extraction procedure allowing to reduce non-specific interactions. In order to test the selectivity of the MIPs, the same procedure was applied to the extraction of compounds possessing the same polarity and size as the triazines. By means of molecular modeling, the effects of the electric charge distribution and of the size of the molecules on the retention mechanism were studied. The value of capacity for terbutylazine MIP was also measured. At last, the high selectivity resulting from the use of MIPs was clearly demonstrated by their applications to the clean-up of grape juice and soil extracts spiked with triazines. In addition, the soil extract was cleaned-up by immunoextraction allowing the comparison of both approaches in terms of selectivity. (C) 2004 Elsevier B.V. All rights reserved.
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页码:93 / 101
页数:9
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