Structure and specific detection of staphylococcal cassette chromosome mec type VII

被引：67

作者：

Higuchi, Wataru ^{[1
]}

Takano, Tomomi ^{[1
]}

Teng, Lee-Jene ^{[2
,3
]}

Yamamoto, Tatsuo ^{[1
]}

机构：

[1] Niigata Univ, Grad Sch Med & Dent Sci, Dept Infect Dis Control & Int Med, Div Bacteriol, Niigata 9518510, Japan

[2] Natl Taiwan Univ Hosp, Taipei, Taiwan

[3] Natl Taiwan Univ, Coll Med, Taipei 10764, Taiwan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 377卷 / 03期

基金：

日本科学技术振兴机构;

关键词：

Methicillin-resistant Staphylococcus aureus (MRSA) Staphylococcal cassette chromosome mec type VII (SCCmecVII); The cassette chromosome recombinase C gene (ccrC); Evolution;

D O I：

10.1016/j.bbrc.2008.10.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Staphylococcal cassette chromosome mec (SCCmec) type VII, found in community-acquired methicillin-resistant Staphylococcus aureus belonging to multilocus sequence type (ST) 59 from Taiwan, was 41,347 bp in size and flanked by 19-bp attL and attR sequences. It was inserted into the att site at the 3'-end of orfX in the orfX-orfY (Putative tRNA dihydrouridine synthase) region in ST59 S. aureus. The 5'-end side 9911-bp core region of SCCmecVII, which contained attL and the cassette chromosome recombinase gene (ccrC8), was shared by other SCC Structures, SCCmercury and mosaic SCCmec from Switzerland, indicating its important role in SCC evolution. The central 21,245-bp core region contained mec complex (C2b) and another ccrC gene (ccrC2), and was highly homologous to SCCmecV. but with substitutions, insertion and replacement. The 3'-end side 10,191-bp sequence was unique. Therefore, SCCmecVII has emerged through recombination and insertion Multiplex and real-time PCR assays were developed for specific detection of SCCmecVII. events. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：752 / 756

页数：5

共 18 条

[1]

Successful multiresistant community-associated methicillin-resistant Staphylococcus aureus lineage from Taipei, Taiwan, that carries either the novel staphylococcal chromosome cassette mec (SCCmec) type VT or SCCmec type IV [J].