Synthesis of plasminogen activator inhibitor 1 by bovine mammary epithelial and myoepithelial cell lines

被引：12

作者：

Zavizion, B

Heegard, CW

White, J

Cheli, F

Politis, I

机构：

[1] AARHUS UNIV,DEPT MOL BIOL,DK-8000 AARHUS C,DENMARK

[2] UNIV MILAN,IST NUTR ANIM,I-20133 MILAN,ITALY

来源：

JOURNAL OF DAIRY RESEARCH | 1996年 / 63卷 / 03期

关键词：

D O I：

10.1017/S0022029900031952

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Conversion of plasminogen to plasmin provides an important source of proteolytic activity in the bovine mammary gland. Plasminogen activator inhibitor 1 (PAI-1.) plays a key role in limiting plasminogen activation. The PAI-1 biosynthetic capabilities of various bovine mammary cells mere determined. The immortalized epithelial cell lines MAC-T and BME-UV and the myoepithelial cell Line BMM-UV were used as model systems. Northern blot analysis indicated that both epithelial and myoepithelial cells contained PAI-1. mRNA. Bovine PAI-1 was encoded by a single mRNA species similar to 3.0 kb long. BME-UV cells contained 2.0-fold (P < 0.01) the PAI-1. mRNA of MAC-T or BMM-UV cells. Reverse zymography indicated that both epithelial and myoepithelial cells synthesized PAI-1 protein with a molecular mass of similar to 50 kDa.

引用

页码：451 / 458

页数：8