Evaluation of a PCR/DNA probe colorimetric membrane assay for identification of Campylobacter spp. in human stool specimens

被引：10

作者：

Collins, E ^{[1
]}

Glennon, M

Hanley, S

Murray, AM

Cormican, M

Smith, T

Maher, M

机构：

[1] Natl Univ Ireland Univ Coll Galway, DNA Diagnost Lab, Natl Diagnost Ctr, BioRes Ireland, Galway, Ireland

[2] Univ Coll Hosp Galway, Dept Med Microbiol, Galway, Ireland

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2001年 / 39卷 / 11期

关键词：

D O I：

10.1128/JCM.39.11.4163-4165.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

DNA was extracted from 50 human stool specimens using the QIAamp DNA stool minikit. PCR amplification was followed by post-PCR hybridization to DNA probes specific for the Campylobacter genus, Campylobacter jejuni, and Campylobacter coli in a colorimetric membrane assay. Thirty-two of 38 culture-positive specimens were PCR/DNA probe positive for C jejuni. The assay is rapid and simple and can be applied to stool specimens for the detection of Campylobacter.

引用

页码：4163 / 4165

页数：3

共 11 条

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