Simultaneous determination of 12 chemical constituents in the traditional Chinese Medicinal Prescription Xiao-Yao-San-Jia-Wei by HPLC coupled with photodiode array detection

被引：43

作者：

Zhang, Hongmin ^{[3
,4
,5
,6
]}

Chen, Shiwei ^{[7
]}

Qjn, Feng ^{[1
,2
]}

Huang, Xi ^{[1
,2
,5
,6
]}

Ren, Ping ^{[1
,2
]}

Gu, Xinqi ^{[5
,6
]}

机构：

[1] Cent S Univ, Xiangya Hosp, Lab Ethnopharmacol, Changsha 410008, Hunan, Peoples R China

[2] Cent S Univ, Xiangya Hosp, Inst Integrated Tradit Med & Western Med, Changsha 410008, Hunan, Peoples R China

[3] Henan Prov Peoples Hosp, Henan Eye Inst, Zhengzhou 450003, Peoples R China

[4] Henan Prov Peoples Hosp, Dept Ophthalmol, Zhengzhou 450003, Peoples R China

[5] Sichuan Univ, W China Hosp, Lab Ethnopharmacol, Chengdu 610041, Peoples R China

[6] Sichuan Univ, W China Hosp, Dept Integrated Chinese & Western Med, Chengdu 610041, Peoples R China

[7] Henan Food & Drug Adm, Zhengzhou 450012, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2008年 / 48卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Xiao-Yao-San-jia-Wei; Traditional Chinese Medicinal Prescription; Chemical constituents; HPLC-PDA;

D O I：

10.1016/j.jpba.2008.09.023

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An HPLC-photodiode array (PDA) detection method was established for the simultaneous determination of 12 components in Xiao-Yao-San-jia-Wei (XYSJW): geniposide, puerarin, paeoniflorin, ferulic acid, liquiritin, hesperidin, naringin, paeonol, daidzein, glycyrrhizic acid, honokiol, and magnolol. These were separated in less than 70 min using a Waters Symmetry Shield RP 18 column with gradient elution using (A) acetonitrile, (B) water, and (C) acetic acid at a flow rate of 1 ml/min, and with a PDA detector. All calibration curves showed good linear regression (r(2) > 0.9992) within the test ranges. The method was validated for specificity, accuracy, precision, and limits of detection. The proposed method enables in a single run the simultaneous identification and determination for quality control of 12 multi-structural components of XYSJW forming the basis of its therapeutic effect. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：1462 / 1466

页数：5

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