Chemical nucleases

被引：361

作者：

Cowan, JA ^{[1
]}

机构：

[1] Ohio State Univ, Evans Lab Chem, Columbus, OH 43210 USA

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2001年 / 5卷 / 06期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/S1367-5931(01)00259-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Substantial progress is being made in the synthesis of functional mimics of metallonuclease enzymes, although a comparison with enzyme-promoted rates of reaction shows considerable room for future improvement. Improved ligand design allows facile substrate binding to metal ion catalysts, while maintaining the stability of the metal complex. The execution of double-strand cleavage of DNA under hydrolytic conditions has been reported and new levels of activity achieved. Finally, considerable progress has been made in identifying the molecular determinants for site-selective cleavage of RNA (in particular) and the design of ligands to achieve targeted recognition and cleavage.

引用

页码：634 / 642

页数：9

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