Evaluation of serotypes of Streptococcus pneumoniae isolated from otitis media patients by multiplex polymerase chain reaction

被引:4
作者
Billal, DS [1 ]
Hotomi, M [1 ]
Tasnim, S [1 ]
Fujihara, K [1 ]
Yamanaka, N [1 ]
机构
[1] Wakayama Med Univ, Dept Otolaryngol Head & Neck Surg, Wakayama 6418509, Japan
来源
ORL-JOURNAL FOR OTO-RHINO-LARYNGOLOGY AND ITS RELATED SPECIALTIES | 2006年 / 68卷 / 03期
关键词
Streptococcus pneumoniae; polymerase chain reaction; serotype; serogroup; acute otitis media;
D O I
10.1159/000091275
中图分类号
R76 [耳鼻咽喉科学];
学科分类号
100213 ;
摘要
The increasing difficulty in the management of pneumococcal acute otitis media in parallel with increases in antimicrobial-resistant strains has led to much interest in pneumococcal capsular types for the adoption of effective prevention by vaccines. This study shows that multiplex polymerase chain reaction is a valuable and expeditious method for the capsular typing of pneumococci. The multiplex polymerase chain reaction method accurately detects the majority of serotypes and serogroups frequently isolated from pediatric patients with acute otitis media, allowing the characterization of the colonization patterns for further implications of pneumococcal vaccines. Copyright (c) 2006 S. Karger AG, Basel.
引用
收藏
页码:135 / 138
页数:4
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