Induction of high-frequency somatic embryogenesis in geranium (Pelargonium x hortorum Bailey cv Ringo Rose) cotyledonary cultures

被引:5
作者
Murthy, BNS [1 ]
Singh, RP [1 ]
Saxena, PK [1 ]
机构
[1] UNIV GUELPH,DEPT HORT SCI,GUELPH,ON N1G 2W1,CANADA
关键词
D O I
暂无
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The cv Ringo Rose of hybrid seed geranium (Pelargonium x hortorum Bailey), previously shown to be recalcitrant in culture, produced somatic embryos when cotyledonary explants were cultured on regeneration medium containing thidiazuron (TDZ), forchlorfenuron (CPPU), or a combination of indole-3-acetic acid and N-6-benzylaminopurine (IAA+BAP). Amendment of the basal medium with TDZ (0.5 mu M) was the most effective treatment. Addition of amino acids to the medium promoted the growth of somatic embryos. Retention of the proximal region of the cotyledon was crucial for regeneration, but the removal of the distal 1/3 to 1/2 cotyledon had no significant effect on somatic embryogenesis. Cotyledonary explants formed somatic embryos in higher frequency and much earlier than hypocotyl explants cultured on the same medium. The somatic embryos induced on cotyledonary explants were germinated on basal medium. More than 70% of the somatic embryos were converted into plants and transferred to soil.
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页码:423 / 426
页数:4
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