Use of bifunctional cross-linking reagents in mapping genomic distribution of chromatin remodeling complexes

被引：29

作者：

Fujita, N ^{[1
]}

Wade, PA ^{[1
]}

机构：

[1] Emory Univ, Sch Med, Dept Pathol & Lab Med, Atlanta, GA 30322 USA

来源：

METHODS | 2004年 / 33卷 / 01期

关键词：

cross-linking; chromatin immunoprecipitation; formaldehyde cross-linking; imidoester cross-linking; protein-DNA cross-linking;

D O I：

10.1016/j.ymeth.2003.10.022

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Chromatin remodeling complexes consist of multiple subunits, some of which are in intimate contact with DNA while others are not. The ability to effectively cross-link proteins to DNA with formaldehyde is impacted by the average distance between the two species. Productive cross-linking of proteins not in direct contact with DNA is greatly facilitated by the inclusion of an initial cross-linking reaction using bifunctional imidoester cross-linking reagents. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：81 / 85

页数：5

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