SYBR green and TaqMan real-time PCR assays are equivalent for the diagnosis of dengue virus type 3 infections

被引：31

作者：

Gomes-Ruiz, AC ^{[1
]}

Nascimento, RT ^{[1
]}

de Paula, SO ^{[1
]}

da Fonseca, BAL ^{[1
]}

机构：

[1] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Internal Med, BR-14049900 Sao Paulo, Brazil

来源：

JOURNAL OF MEDICAL VIROLOGY | 2006年 / 78卷 / 06期

关键词：

dengue; real-time PCR; TaqMan; Sybr Green;

D O I：

10.1002/jmv.20620

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学]; 100705 [微生物与生化药学];

摘要：

Dengue is the most important arthropod-borne viral disease in the world. A rapid diagnostic test for dengue is warranted, and real-time polymerase chain reaction may improve diagnosis. Taq-Man and Sybr Green systems were evaluated for the diagnosis of dengue virus type 3 (DENV-3) infections. Out of 77 patients with clinical suspicion of dengue infection, specific IgM antibodies were detected in 40 patients. DENV-3 was detected and quantitated in 17 IgM-positive samples by both systems. These assays were shown to be rapid, and specific for detection of DENV-3, and that for diagnostic purposes, there is no difference between these two assays.

引用

页码：760 / 763

页数：4

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