Evaluation of cholecystokinin, gastrin, CCK-A receptor, and CCK-B/gastrin receptor gene expressions in gastric cancer

被引：32

作者：

Okada, N ^{[1
]}

Kubota, A ^{[1
]}

Imamura, T ^{[1
]}

Suwa, H ^{[1
]}

Kawaguchi, Y ^{[1
]}

Ohshio, G ^{[1
]}

Seino, Y ^{[1
]}

Imamura, M ^{[1
]}

机构：

[1] KYOTO UNIV, FAC MED, DEPT METAB & CLIN NUTR, SAKYO KU, KYOTO 606, JAPAN

来源：

CANCER LETTERS | 1996年 / 106卷 / 02期

关键词：

cholecystokinin (CCK); gastrin; CCK-A receptor; CCK-B receptor; reverse transcription polymerase chain reaction;

D O I：

10.1016/0304-3835(96)04325-X

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The brain-gut hormones, cholecystokinin (CCK) and gastrin, regulate the growth of gastrointestinal mucosa and tumor cells. In this study, reverse transcription-polymerase chain reaction (RT-PCR) was used to evaluate messenger RNA expression for CCK, gastrin, CCK-A receptor, and CCK-B/gastrin receptor in surgical specimens of gastric cancers and in normal antrum and body mucosa of the stomach. The CCK mRNA expression was detectable in 4/14 (29%) samples of gastric cancer and in 3/12 (25%)samples of antral mucosa. However, the gastrin mRNA expression was not detectable in any gastric cancer samples, although it was detectable in all the samples of antral mucosa. The CCK-A receptor mRNA expression was detectable in 5/14 (36%) samples of gastric cancer and in 7/12 (58%) body mucosa. Three cases out of 14 (21%) of gastric cancer expressed both CCK gene and CCK-A receptor gene-The CCK-B receptor mRNA expression was detectable in only 1/14 (7%) samples of gastric cancer, although it was detectable in 10/12 (83%) body mucosa of the stomach. These findings may suggest a greater role for CCK and CCK-A receptor than for gastrin and CCK-B receptor in gastric cancers.

引用

页码：257 / 262

页数：6

共 27 条

[21] GASTROINTESTINAL HORMONES AND GASTROINTESTINAL AND PANCREATIC CARCINOMAS [J].

TOWNSEND, CM ;

SINGH, P ;

THOMPSON, JC .

GASTROENTEROLOGY, 1986, 91 (04) :1002-1006

[22]

UPP JR, 1989, CANCER RES, V49, P488

[23] COTRANSCRIPTION OF THE GASTRIN AND CHOLECYSTOKININ GENES WITH SELECTIVE TRANSLATION OF GASTRIN MESSENGER-RNA IN A HUMAN GASTRIC-CARCINOMA CELL-LINE [J].

VANSOLINGE, WW ;

REHFELD, JF .

FEBS LETTERS, 1992, 309 (01) :47-50

[24] GROWTH-PROMOTING ACTION OF GASTRIN ON HUMAN COLONIC AND GASTRIC TUMOR-CELLS CULTURED INVITRO [J].

WATSON, SA ;

DURRANT, LG ;

MORRIS, DL .

BRITISH JOURNAL OF SURGERY, 1988, 75 (04) :342-345

[25] THE INVITRO GROWTH-RESPONSE OF PRIMARY HUMAN COLORECTAL AND GASTRIC-CANCER CELLS TO GASTRIN [J].

WATSON, SA ;

DURRANT, LG ;

CROSBIE, JD ;

MORRIS, DL .

INTERNATIONAL JOURNAL OF CANCER, 1989, 43 (04) :692-696

[26]

WEINSTOCK J, 1988, CANCER RES, V48, P932

[27]

WIORG O, 1984, P NATL ACAD SCI USA, V81, P1067

← 1 2 3 →