Establishment of a non-tumorigenic papillary thyroid cell line (FB-2) carrying the RET/PTC1 rearrangement

被引：39

作者：

Basolo, F

Giannini, R

Toniolo, A

Casalone, R

Nikiforova, M

Pacini, F

Elisei, R

Miccoli, P

Berti, P

Faviana, P

Fiore, L

Monaco, C

Pierantoni, GM

Fedele, M

Nikiforov, YE

Santoro, M

Fusco, A

机构：

[1] Univ Pisa, Div Pathol, Dept Oncol, I-56126 Pisa, Italy

[2] Univ Pisa, Ist Endocrinol, Pisa, Italy

[3] Univ Pisa, Dipartimento Chirurg, Pisa, Italy

[4] Univ Insubria, Dipartimento Sci Clin & Biol, Varese, Italy

[5] Univ Naples Federico II, Dipartimento Biol & Patol Cellulare & Mol, CNR, Ctr Endocrinol & Oncol Sperimentale, Naples, Italy

[6] Univ Cincinnati, Dept Pathol, Cincinnati, OH USA

来源：

INTERNATIONAL JOURNAL OF CANCER | 2002年 / 97卷 / 05期

关键词：

thyroid; papillary carcinoma; cell line; RET rearrangement;

D O I：

10.1002/ijc.10116

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A novel human thyroid papillary carcinoma cell line (FB-2) has been established and characterized. FB-2 cells harbor the RET/PTCI chimeric oncogene in which the RET kinase domain is fused to the H4 gene. FB-2 cells neither formed colonies in semisolid media nor induced tumors after heterotransplant into severe combined immunodeficient mice. However, HMGI(Y), HMGI-C and c-myc genes, which are associated to thyroid cell transformation, were abundantly expressed in FB-2 cells but not in normal thyroid cells. FB-2 cells only partially retained the differentiated thyroid phenotype. In fact, the PAX-8 gene, which codes for a transcriptional factor required for thyroid cell differentiation, was expressed, while thyroglobulin, TSH-receptor and thyroperoxidase genes were not. Moreover, FB-2 cells produced high levels of interleukin (IL)-6 and IL-8. (C) 2002 Wiley-Liss, Inc.

引用

页码：608 / 614

页数：7

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