Production and extraction of pectinases obtained by solid state fermentation of agroindustrial residues with Aspergillus niger

被引:151
作者
Castilho, LR
Medronho, RA
Alves, TLM
机构
[1] Fed Univ Rio De Janeiro, Program Chem Engn, COPPE, Ctr Tecnol, BR-21945970 Rio De Janeiro, Brazil
[2] Univ Fed Rio de Janeiro, Sch Chem, Ctr Tecnol, BR-21949900 Rio De Janeiro, Brazil
关键词
pectinases; Aspergillus niger; wheat bran; soy bran; humidity; extraction;
D O I
10.1016/S0960-8524(99)00058-9
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
In this work soy and wheat bran were employed as raw materials for the production of pectinases by Aspergillus niger through solid-state fermentation. Several fermentation and recovery parameters were studied. The kinetics of enzyme synthesis was investigated in the range from 13 to 96 h with moisture contents varying from 25% to 70% (w/w). A medium moisture content of 40% and a fermentation time of 22 h were selected, as these conditions resulted in high pectolytic activity and enhanced polygalacturonase productivity. In order to optimise the recovery step, the best combination of temperature of extraction, contact time and solvent type was investigated. Acetate buffer (pH 4.4), 35 degrees C and 30 min provided the best recovery. The present results !;how that optimising the extraction conditions is a simple way of obtaining more concentrated enzyme extracts and could be a useful instrument to extract more selectively a desired biomolecule from fermented solids. (C) 1999 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:45 / 50
页数:6
相关论文
共 27 条
[1]   EFFECT OF WATER ACTIVITY ON EXO-PECTINASE PRODUCTION BY ASPERGILLUS-NIGER CH4 ON SOLID-STATE FERMENTATION [J].
ACUNAARGUELLES, M ;
GUTIERREZROJAS, M ;
VINIEGRAGONZALEZ, G ;
FAVELATORRES, E .
BIOTECHNOLOGY LETTERS, 1994, 16 (01) :23-28
[2]  
ACUNAARGUELLES ME, 1995, APPL MICROBIOL BIOT, V43, P808, DOI 10.1007/s002530050489
[3]   Xylanase production by thermophilic Bacillus licheniformis A99 in solid-state fermentation [J].
Archana, A ;
Satyanarayana, T .
ENZYME AND MICROBIAL TECHNOLOGY, 1997, 21 (01) :12-17
[4]   CULTURE REQUIREMENTS FOR THE PRODUCTION OF PROTEASE BY ASPERGILLUS-ORYZAE IN SOLID-STATE FERMENTATION [J].
BATTAGLINO, RA ;
HUERGO, M ;
PILOSOF, AMR ;
BARTHOLOMAI, GB .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1991, 35 (03) :292-296
[5]  
BEROVIC M, 1993, J CHEM TECHNOL BIOT, V56, P209
[6]   Recovery of pectolytic enzymes produced by solid state culture of Aspergillus niger [J].
Castilho, LR ;
Alves, TLM ;
Medronho, RA .
PROCESS BIOCHEMISTRY, 1999, 34 (02) :181-186
[7]  
CASTILHO LR, 1997, THESIS COPPE FEDERAL
[8]  
CASTILHO LR, 1996, P 11 BRAZ C CHEM ENG, V2, P1466
[9]  
Coelho MAZ, 1995, REV MICROBIOL, V26, P318
[10]  
Couri S, 1995, REV MICROBIOL, V26, P314