Recovery of pectolytic enzymes produced by solid state culture of Aspergillus niger

被引:47
作者
Castilho, LR
Alves, TLM
Medronho, RA
机构
[1] Univ Fed Rio de Janeiro, COPPE, Program Chem Engn, Ctr Tecnol, BR-21949900 Rio De Janeiro, Brazil
[2] Univ Fed Rio de Janeiro, Sch Chem, Dept Chem Engn, Ctr Tecnol, BR-21949900 Rio De Janeiro, Brazil
关键词
pectinases; polygalacturonase; recovery; extraction; solid state fermentation; Aspergillus niger;
D O I
10.1016/S0032-9592(98)00089-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Pectinases are enzymes with a wide range of applications in the food and drink industries. In the present work, the extraction of pectinases produced by Aspergillus niger in a solid state fermentation system was investigated. The purpose was to reduce enzyme losses in the fermented solids and at the same time obtain a crude extract as concentrated as possible. Initially the performances of stirred tank and fixed bed extractors were compared. Polygalacturonase activity and viscosity reducing capacity obtained in the stirred tank system were 105% and 15% superior, respectively. Repeated extractions and multiple stage countercurrent extraction were studied, employing stirred tanks. It was possible to observe that three stages were enough for total recovery of the enzymes contained in the solids. The final enzyme extract obtained by counter-current extraction with three stages showed a polygalacturonase activity 81% higher than the one obtained by one-stage extraction. (C) 1999 Published by Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:181 / 186
页数:6
相关论文
共 13 条
[1]  
CASTILHO LR, 1997, THESIS FEDERAL U RIO
[2]  
CASTILHO LR, 1996, P 11 BRAZ C CHEM ENG, V2, P1466
[3]  
Coelho MAZ, 1995, REV MICROBIOL, V26, P318
[4]  
COONEY CL, 1987, DOWNSTREAM PROCESSIN
[5]  
Couri S, 1995, REV MICROBIOL, V26, P314
[6]   EFFICIENT AND SIMPLE EXTRACTION OF MOLDY BRAN IN A PULSED COLUMN EXTRACTOR FOR RECOVERY OF AMYLOGLUCOSIDASE IN CONCENTRATED FORM [J].
GHILDYAL, NP ;
RAMAKRISHNA, M ;
LONSANE, BK ;
KARANTH, NG .
PROCESS BIOCHEMISTRY, 1991, 26 (04) :235-241
[7]  
KUMAR PKR, 1987, PROCESS BIOCHEM, V22, P139
[8]  
LANGONE MAP, 1996, BRAZ J CHEM ENG, V13, P1
[9]  
McCabe W.L., 1985, UNIT OPERATIONS CHEM
[10]   Extraction and purification of amyloglucosidase produced by solid state fermentation with Aspergillus niger [J].
Ramadas, M ;
Holst, O ;
Mattiasson, B .
BIOTECHNOLOGY TECHNIQUES, 1995, 9 (12) :901-906