Antiviral activity of polysaccharide extract from Laminaria japonica against respiratory syncytial virus

被引：51

作者：

Cao, Yin-guang ^{[1
,2
]}

Hao, Yu ^{[1
]}

Li, Zhi-hui ^{[1
,2
]}

Liu, Shun-tao ^{[1
]}

Wang, Le-xin ^{[3
]}

机构：

[1] Taishan Med Univ, Clin Lab, Liaocheng Peoples Hosp, Tai An 252000, Shandong, Peoples R China

[2] Shandong Univ, Sch Med, Jinan 250012, Shandong, Peoples R China

[3] Charles Sturt Univ, Sch Biomed Sci, Wagga Wagga, NSW 2678, Australia

来源：

BIOMEDICINE & PHARMACOTHERAPY | 2016年 / 84卷

关键词：

Laminaria japonica; Respiratory syncytial virus; Polysaccharide extract; Interferon-alpha; Antiviral activity; ANTI-RSV; INHIBITOR; DISCOVERY; FUCOIDAN; GENE; MICE;

D O I：

10.1016/j.biopha.2016.10.082

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

100103 [病原生物学]; 100218 [急诊医学];

摘要：

This study was designed to investigate the inhibition activity of polysaccharide extract from Laminaria japonica against RSV. The polysaccharide from Laminaria japonica was isolated by ethanol precipitation. HEK293 cells were infected with RVS, and the antiviral activity of polysaccharide extract against RSV in host cells was tested. By using ELISA and western blot assay, the expression level of IFN-alpha and IRF3 and their functional roles in polysaccharide-mediated antiviral activity against RSV were investigated. The polysaccharide extract from Laminaria japonica had low toxicity to HEK293 cell. The TC50 to HEK293 cells was up to 1.76 mg/mL. Furthermore, the EC50 of polysaccharide extract to RSV was 5.27 mu g/mL, and TI was 334. The polysaccharide extract improved IRF-3 expression which promoted the level of IFN-alpha. In conclusion: Polysaccharide extract from Laminaria japonica elicits antiviral activity against RSV by upregulation of IRF3 signaling-mediated IFN-a production. (C) 2016 Elsevier Masson SAS. All rights reserved.

引用

页码：1705 / 1710

页数：6

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