Padlock oligonucleotides for duplex DNA based on sequence-specific triple helix formation

被引：50

作者：

Escudé, C ^{[1
]}

Garestier, T ^{[1
]}

Hélène, C ^{[1
]}

机构：

[1] Museum Natl Hist Nat, Biophys Lab, INSERM U201, CNRS UMR 8646, F-75231 Paris, France

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1999年 / 96卷 / 19期

关键词：

D O I：

10.1073/pnas.96.19.10603

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An oligonucleotide was circularized around double-stranded DNA thanks to triple helix formation. Short oligonucleotides are known to be able to form DNA triple helices by binding into the DNA major groove at an oligopurine oligopyrimidine sequence. After sequence-specific recognition of a double-stranded DNA target through triple helix formation, the ends of the tripler-forming oligonucleotide were joined through the action of T4 DNA ligase, thus creating a circular DNA molecule catenated to the plasmid containing the target sequence. The labeling of the double-stranded DNA sequence has been carried out without any chemical or enzymatic modification of this sequence. These "padlock" oligonucleotides provide a tool to attach a noncovalent tag in an irreversible way to supercoiled plasmid or other double-stranded DNAs. Such a complex may find applications in the development of new techniques for duplex DNA detection or plasmid delivery methods for gene therapy.

引用

页码：10603 / 10607

页数：5

共 25 条

[1] Signal amplification of padlock probes by rolling circle replication [J].

Banér, J ;

Nilsson, M ;

Mendel-Hartvig, M ;

Landegren, U .

NUCLEIC ACIDS RESEARCH, 1998, 26 (22) :5073-5078

[2]

Cheng L, 1996, BIOTECHNIQUES, V21, P486

[3] Coupling of nuclear localization signals to plasmid DNA and specific interaction of the conjugates with importin α [J].

Ciolina, C ;

Byk, G ;

Blanche, F ;

Thuillier, V ;

Scherman, D ;

Wils, P .

BIOCONJUGATE CHEMISTRY, 1999, 10 (01) :49-55

[4] Ligand-induced formation of triple helices with antiparallel third strands containing G and T [J].

Escude, C ;

Sun, JS ;

Nguyen, CH ;

Bisagni, E ;

Garestier, T ;

Helene, C .

BIOCHEMISTRY, 1996, 35 (18) :5735-5740

[5] ROLLING REPLICATION OF SHORT DNA CIRCLES [J].

FIRE, A ;

XU, SQ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (10) :4641-4645

[6] SEQUENCE-SPECIFIC RECOGNITION AND CLEAVAGE OF DUPLEX DNA VIA TRIPLE-HELIX FORMATION BY OLIGONUCLEOTIDES COVALENTLY LINKED TO A PHENANTHROLINE COPPER CHELATE [J].

FRANCOIS, JC ;

SAISONBEHMOARAS, T ;

BARBIER, C ;

CHASSIGNOL, M ;

THUONG, NT ;

HELENE, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (24) :9702-9706

[7] Accessibility of nuclear DNA to triplex-forming oligonucleotides: The integrated HIV-1 provirus as a target [J].

Giovannangeli, C ;

Diviacco, S ;

Labrousse, V ;

Gryaznov, S ;

Charneau, P ;

Helene, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (01) :79-84

[8] Recognition of DNA, RNA, and proteins by circular oligonucleotides [J].

Kool, ET .

ACCOUNTS OF CHEMICAL RESEARCH, 1998, 31 (08) :502-510

[9]

Kuhn H, 1999, ANGEW CHEM INT EDIT, V38, P1446, DOI 10.1002/(SICI)1521-3773(19990517)38:10<1446::AID-ANIE1446>3.0.CO

[10]

2-3

← 1 2 3 →