Whole-organism fingerprinting of the genus Carnobacterium using Fourier transform infrared spectroscopy (FT-IR)

被引：13

作者：

Lai, S ^{[1
]}

Goodacre, R ^{[1
]}

Manchester, LN ^{[1
]}

机构：

[1] Univ Wales, Inst Biol Sci, Aberystwyth SY23 3DA, Dyfed, Wales

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 2004年 / 27卷 / 02期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Carnobacterium; taxonomy; Fourier transform infrared spectroscopy;

D O I：

10.1078/072320204322881808

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Sixty seven strains of Carnobacterium, atypical Lactobacillus, Enterococcus durans, Lactobacillus maltaromicus and Vagacoccus salmoninarum were examined by Fourier transform infrared (FT-IR) spectroscopy. The effects of culture age and reproducibility over a six month period were also investigated. The results were analysed by multivariate statistics and compared with those from a previous numerical phenetic study, a pyrolysis mass spectrometry (PyMS) study and with investigations which used DNA-DNA and 16S rRNA sequencing homologies. Taxonomic correlations were observed between the FT-IR data and these studies. Culture age was observed to have little effect on the spectra obtained. The reproducibility study indicated that there was correlation between spectra produced on two occasions over the six month period. It was concluded that FTIR is a reliable method for investigating carnobacterial classification, and may have further potential as a rapid method for use in Carnobacterium identification.

引用

页码：186 / 191

页数：6

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