Immobilization induces alterations in the outer membrane protein pattern of Yersinia ruckeri

被引:17
作者
Coquet, L
Cosette, P
Dé, E
Galas, L
Vaudry, H
Rihouey, C
Lerouge, P
Junter, GA
Jouenne, T [1 ]
机构
[1] Univ Rouen, Fac Sci, CNRS, UMR 6522,IBBR Grp,Lab Polymeres Biopolymeres Memb, F-76821 Mont St Aignan, France
[2] Univ Rouen, CNRS, INSERM, Lab Cellular & Mol Neuroendocrinol,INSERM,U413, F-76821 Mont St Aignan, France
[3] Univ Rouen, CNRS, UMR 6037, F-76821 Mont St Aignan, France
关键词
biofilm; proteome; outer membrane; immobilized cells; pH;
D O I
10.1021/pr050165c
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We compared the outer membrane protein (OMP) pattern of 2-day-old immobilized Yersinia ruckeri cells (IC) with that of early (FC24) and late (FC48) stationary-phase planktonic counterparts. Fifty-five OMPs were identified. Principal component analysis discriminated between the protein maps of FC and IC. Some OMPs involved in bacterial adaptation were accumulated by both FC48 and IC but the expression of other proteins was controlled by the sessile mode of growth.
引用
收藏
页码:1988 / 1998
页数:11
相关论文
共 69 条
[21]   Immobilized-cell physiology: current data and the potentialities of proteomics [J].
Junter, GA ;
Coquet, L ;
Vilain, S ;
Jouenne, T .
ENZYME AND MICROBIAL TECHNOLOGY, 2002, 31 (03) :201-212
[22]   TYROSINE PHOSPHATE IN A-TYPE AND B-TYPE FLAGELLINS OF PSEUDOMONAS-AERUGINOSA [J].
KELLYWINTENBERG, K ;
SOUTH, SL ;
MONTIE, TC .
JOURNAL OF BACTERIOLOGY, 1993, 175 (08) :2458-2461
[23]   Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants [J].
Klausen, M ;
Heydorn, A ;
Ragas, P ;
Lambertsen, L ;
Aaes-Jorgensen, A ;
Molin, S ;
Tolker-Nielsen, T .
MOLECULAR MICROBIOLOGY, 2003, 48 (06) :1511-1524
[24]   Carbapenem activities against Pseudomonas aeruginosa:: Respective contributions of OprD and efflux systems [J].
Köhler, T ;
Michea-Hamzehpour, M ;
Epp, SF ;
Pechere, JC .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (02) :424-427
[25]   Surface-induced and biofilm-induced changes in gene expression [J].
Kuchma, SL ;
O'Toole, GA .
CURRENT OPINION IN BIOTECHNOLOGY, 2000, 11 (05) :429-433
[26]   A novel two-component system controls the expression of Pseudomonas aeruginosa fimbrial cup genes [J].
Kulasekara, HD ;
Ventre, I ;
Kulasekara, BR ;
Lazdunski, A ;
Filloux, A ;
Lory, S .
MOLECULAR MICROBIOLOGY, 2005, 55 (02) :368-380
[27]   OPTICAL SECTIONING OF MICROBIAL BIOFILMS [J].
LAWRENCE, JR ;
KORBER, DR ;
HOYLE, BD ;
COSTERTON, JW ;
CALDWELL, DE .
JOURNAL OF BACTERIOLOGY, 1991, 173 (20) :6558-6567
[28]   CHARACTERIZATION AND ENVIRONMENTAL-REGULATION OF OUTER-MEMBRANE PROTEINS IN XENORHABDUS-NEMATOPHILUS [J].
LEISMAN, GB ;
WAUKAU, J ;
FORST, SA .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (01) :200-204
[29]   Contamination of abiotic surfaces: what a colonizing bacterium sees and how to blur it [J].
Lejeune, P .
TRENDS IN MICROBIOLOGY, 2003, 11 (04) :179-184
[30]   A phosphate-starvation-inducible outer-membrane protein of Pseudomonas fluorescens Ag1 as an immunological phosphate-starvation marker [J].
Leopold, K ;
Jacobsen, S ;
Nybroe, O .
MICROBIOLOGY-SGM, 1997, 143 :1019-1027