NMR-based structural characterization of large protein-ligand interactions

被引：91

作者：

Pellecchia, M ^{[1
]}

Meininger, D ^{[1
]}

Dong, Q ^{[1
]}

Chang, E ^{[1
]}

Jack, R ^{[1
]}

Sem, DS ^{[1
]}

机构：

[1] Triad Therapeut Inc, San Diego, CA 92121 USA

来源：

JOURNAL OF BIOMOLECULAR NMR | 2002年 / 22卷 / 02期

关键词：

drug design; drug discovery; ligand binding; ligand docking; NMR; protein structure;

D O I：

10.1023/A:1014256707875

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genomic research on target identification and validation has created a great need for methods that rapidly provide detailed structural information on protein-ligand interactions. We developed a suite of NMR experiments as rapid and efficient tools to provide descriptive structural information on protein-ligand complexes. The methods work with large proteins and in particular cases also without the need for a complete three-dimensional structure. We will show applications with two tetrameric enzymes of 120 and 170 kDa.

引用

页码：165 / 173

页数：9

共 28 条

[21] Characterization of the binding interface between the E-domain of Staphylococcal protein A and an antibody Fv-fragment
Meininger, DP
Rance, M
Starovasnik, MA
Fairbrother, WJ
Skelton, NJ
[J]. BIOCHEMISTRY, 2000, 39 (01) : 26 - 36
[22] Incorporation of H-1/C-13/N-15-{Ile, Leu, Val} into a perdeuterated, N-15-labeled protein: Potential in structure determination of large proteins by NMR
Metzler, WJ
Wittekind, M
Goldfarb, V
Mueller, L
Farmer, BT
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (28) : 6800 - 6801
[23] NEUHAUS D, 2000, NUCL OVERHAUSER EFFE, P129
[24] SCOPIN G, 1997, BIOCHEMISTRY-US, V36, P15081
[25] Sem D S, 2001, Curr Opin Drug Discov Devel, V4, P479
[26] COMPUTER-OPTIMIZED DECOUPLING SCHEME FOR WIDEBAND APPLICATIONS AND LOW-LEVEL OPERATION
SHAKA, AJ
BARKER, PB
FREEMAN, R
[J]. JOURNAL OF MAGNETIC RESONANCE, 1985, 64 (03) : 547 - 552
[27] Global efforts in structural genomics
Stevens, RC
Yokoyama, S
Wilson, IA
[J]. SCIENCE, 2001, 294 (5540) : 89 - 92
[28] Measurement of cross-relaxation between amide protons in N-15-enriched proteins with suppression of spin diffusion
Vincent, SJF
Zwahlen, C
Bolton, PH
Logan, TM
Bodenhausen, G
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (14) : 3531 - 3532

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