Branching pattern of gluco-oligosaccharides and 1.5 kDa dextran grafted by the α-1,2 branching sucrase GBD-CD2

被引：13

作者：

Brison, Yoann ^{[1
,2
,3
,4
,5
]}

Laguerre, Sandrine ^{[1
,2
,3
,4
,5
]}

Lefoulon, Francois ^{[6
]}

Morel, Sandrine ^{[1
,2
,3
,4
,5
]}

Monties, Nelly ^{[1
,2
,3
,4
,5
]}

Potocki-Veronese, Gabrielle ^{[1
,2
,3
,4
,5
]}

Monsan, Pierre ^{[1
,2
,3
,4
,5
]}

Remaud-Simeon, Magali ^{[1
,2
,3
,4
,5
]}

机构：

[1] Univ Toulouse, F-31077 Toulouse, France

[2] INSA, UPS, INP, F-31077 Toulouse, France

[3] LISBP, F-31077 Toulouse, France

[4] CNRS, UMR5504, F-31400 Toulouse, France

[5] INRA, Ingn Syst Biol & Proc UMR792, F-3140 Toulouse, France

[6] Technol Servier, F-45000 Orleans, France

来源：

CARBOHYDRATE POLYMERS | 2013年 / 94卷 / 01期

关键词：

Glucansucrase; alpha-(1 -> 2) Branching sucrase; Sucrose; Gluco-oligosaccharides; Dextran; Prebiotics; MESENTEROIDES NRRL B-1299; IN-VITRO FERMENTATION; LACTIC-ACID BACTERIA; 2 CATALYTIC DOMAINS; HIGH-FAT DIET; DSR-E; STRUCTURAL-CHARACTERIZATION; MOLECULAR-WEIGHT; GLUCANSUCRASE; GLUCOSYLTRANSFERASES;

D O I：

10.1016/j.carbpol.2013.01.064

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

GBD-CD2, an engineered sucrose-acting enzyme of glycoside hydrolase family 70, transfers D-glucopyranosyl (D-Glcp) units from sucrose onto dextrans or gluco-oligosaccharides (GOS) through the formation of alpha-(1 -> 2) linkages leading to branched products of interest for health, food and cosmetic applications. Structural characterization of the branched products obtained from sucrose and pure GOS of degree of polymerization (DP) 4 or DP 5 revealed that highly alpha-(1 -> 2) branched and new molecular structures can be synthesized by GBD-CD2. The formation of alpha-(1 -> 2) branching is kinetically controlled and can occur onto vicinal alpha-(1 -> 6)-linked D-Glcp residues. To investigate the mode of branching of 1.5 kDa dextran, simulations of various branching scenarios and resistance to glucoamylase degradation were performed. Analysis of the simulation results suggests that the branching process is stochastic and indicates that the enzyme acceptor site can accommodate both linear and poly-branched acceptors. This opens the way to the design of novel enzyme-based processes yielding carbohydrate structures varying in size and resistance to hydrolytic enzymes. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：567 / 576

页数：10

共 33 条

[1]

Andre I., 2010, SUCROSE UTILIZING TR, VI, P1

[2] Leuconostoc mesenteroides glucansucrase synthesis of flavonoid glucosides by acceptor reactions in aqueous-organic solvents [J].