Fingerprint analysis of Flos Lonicerae japonicae using binary HPLC profiling

被引：33

作者：

Li, Famei ^{[1
]}

Yuan, Bo ^{[1
]}

Xiong, Zhili ^{[1
]}

Lu, Xiumei ^{[1
]}

Qin, Feng ^{[1
]}

Chen, Huashan ^{[1
]}

Liu, Zhigang ^{[1
]}

机构：

[1] Shenyang Pharmaceut Univ, Dept Analyt Chem, Shenyang 110016, Peoples R China

来源：

BIOMEDICAL CHROMATOGRAPHY | 2006年 / 20卷 / 6-7期

关键词：

Flos L. japonicae; chromatographic profiling; chromatographic fingerprinting; in vitro bacteriostasis activity;

D O I：

10.1002/bmc.678

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Binary chromatographic profiling was employed in fingerprint analysis of Flos Lonicerae japonicae, the flower bud of Lonicera japonica Thunb. Standardized procedures were used to develop the profiling for both aqueous and ethanolic extracts. Other species in the previous Chinese Pharmacopoeia and some in folk remedy were successfully differentiated from L. Japonica Thunb. by hierarchiral cluster analysis of the chromatographic profiles. Correlation analysis showed that six chromatographic peaks in ethanolic extract were positively correlated with in vitro bacteriostasis activity. Two standard fingerprints were developed with 10 genuine samples of L. japonica Thunb. Similarity analysis with a limited number of samples showed a fair consistence in the chromatographic profiling of L. japonica Thunb. from various sources and two harvests, and significant differences from other species. Combination use of the two fingerprints demonstrated confirmative identification and quality assessment of Flos Lonicerae japonicae. Copyright (c) 2006 John Wiley & Sons, Ltd.

引用

页码：634 / 641

页数：8

共 34 条

[1]

[Anonymous], GEN GUID METH RES EV

[2] PROGRESS IN TRADITIONAL CHINESE MEDICINE [J].

CHAN, K .

TRENDS IN PHARMACOLOGICAL SCIENCES, 1995, 16 (06) :182-187

[3] Fractal fingerprinting of chromatographic profiles based on wavelet analysis and its application to characterize the quality grade of medicinal herbs [J].

Cheng, YY ;

Chen, MJ ;

Welsh, WJ .

JOURNAL OF CHEMICAL INFORMATION AND COMPUTER SCIENCES, 2003, 43 (06) :1959-1965

[4] Preprocessing of HPLC trace impurity patterns by wavelet packets for pharmaceutical fingerprinting using artificial neural networks [J].

Collantes, ER ;

Duta, R ;

Welsh, WJ ;

Zielinski, WL ;

Brower, J .

ANALYTICAL CHEMISTRY, 1997, 69 (07) :1392-1397

[5] Trends in alternative medicine use in the United States, 1990-1997 - Results of a follow-up national survey [J].

Eisenberg, DM ;

Davis, RB ;

Ettner, SL ;

Appel, S ;

Wilkey, S ;

van Rompay, M ;

Kessler, RC .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1998, 280 (18) :1569-1575

[6]

*EMA, 2001, NOT GUID QUAL HERB M, P6

[7]

*FDA, 2000, GUID IND BOT DRUG PR, pE2

[8] Information theory applied to chromatographic fingerprint of herbal medicine for quality control [J].

Gong, F ;

Liang, YZ ;

Xie, PS ;

Chau, FT .

JOURNAL OF CHROMATOGRAPHY A, 2003, 1002 (1-2) :25-40

[9] Fingerprinting of Salvia miltiorrhiza Bunge by non-aqueous capillary electrophoresis compared with high-speed counter-current chromatography [J].

Gu, M ;

Zhang, SF ;

Su, ZG ;

Chen, Y ;

Fan, OY .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1057 (1-2) :133-140

[10] Identification of major active constituents in the fingerprint of Salvia miltiorrhiza Bunge developed by high-speed counter-current chromatography [J].

Gu, M ;

Zhang, GF ;

Su, ZG ;

Fan, OY .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1041 (1-2) :239-243

← 1 2 3 4 →