Identification of major active constituents in the fingerprint of Salvia miltiorrhiza Bunge developed by high-speed counter-current chromatography

被引：93

作者：

Gu, M ^{[1
]}

Zhang, GF ^{[1
]}

Su, ZG ^{[1
]}

Fan, OY ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Proc Engn, Beijing 100080, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1041卷 / 1-2期

关键词：

Salvia miltiorrhiza; counter-current chromatography; fingerprinting; pharmaceutical analysis; cryptotanshinone; tanshinones;

D O I：

10.1016/j.chroma.2004.04.030

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-speed counter-current chromatography was applied as a method to develop fingerprinting of Salvia miltiorrhiza Bunge, a popular traditional Chinese medicine, in our previous study. Important active constituents that were directly related to the therapy effect should be identified. Each effluent fraction and standard samples (cryptotanshinone, tanshinoneI and tanshinoneIIA) were analyzed by ultraviolet spectroscopy and liquid chromatography-mass spectroscopy. It was concluded from the UV-Vis spectrograms, retention times in LC analysis and mass spectrograms, that fractions 7, 8 and 11 were respectively cryptotanshinone (M-r 296), tanshinone I (M-r 276) and tanshinone IIA (M-r 294). (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：239 / 243

页数：5

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