Histological and SEM analysis of root cementum following irradiation with Er:YAG and CO2 lasers

被引:38
作者
Almehdi, Aslam [1 ,2 ]
Aoki, Akira [1 ]
Ichinose, Shizuko [3 ]
Taniguchi, Yoichi [1 ]
Sasaki, Katia M. [4 ]
Ejiri, Kenichiro [1 ]
Sawabe, Masanori [1 ]
Chui, Chanthoeun [1 ,2 ]
Katagiri, Sayaka [1 ]
Izumi, Yuichi [1 ,2 ]
机构
[1] Tokyo Med & Dent Univ, Dept Periodontol, Grad Sch Med & Dent Sci, Bunkyo Ku, Tokyo 1138549, Japan
[2] Tokyo Med & Dent Univ, Global Ctr Excellence GCOE Program, Int Res Ctr Mol Sci Tooth & Bone Dis, Tokyo 1138549, Japan
[3] Tokyo Med & Dent Univ, Res Ctr Med & Dent Sci, Tokyo 1138549, Japan
[4] Univ Brasilia, Dept Dent, Brasillia, Brazil
关键词
Cementum; Alteration; Microstructure; Er:YAG laser; CO2; laser; Ultrasonic scaler; ER-YAG LASER; NONSURGICAL PERIODONTAL THERAPY; DENTAL HARD SUBSTANCES; IN-VITRO; SUBGINGIVAL CALCULUS; CLINICAL-EVALUATION; SURFACES; TISSUE; VIVO; ABLATION;
D O I
10.1007/s10103-012-1110-7
中图分类号
R318 [生物医学工程];
学科分类号
100103 [病原生物学];
摘要
Recently, the Er:YAG and CO2 lasers have been applied in periodontal therapy. However, the characteristics of laser-irradiated root cementum have not been fully analyzed. The aim of this study was to precisely analyze the alterations of root cementum treated with the Er:YAG and the CO2 lasers, using non-decalcified thin histological sections. Eleven cementum plates were prepared from extracted human teeth. Pulsed Er:YAG laser contact irradiation was performed in a line at 40 mJ/pulse (14.2 J/cm(2)/pulse) and 25 Hz (1.0 W) under water spray. Continuous CO2 laser irradiation was performed in non-contact mode at 1.0 W, and ultrasonic instrumentation was performed as a control. The treated samples were subjected to stereomicroscopy, scanning electron microscopy (SEM), light microscopy and SEM energy dispersive X-ray spectroscopy (SEM-EDS). The Er:YAG laser-treated cementum showed minimal alteration with a whitish, slightly ablated surface, whereas CO2 laser treatment resulted in distinct carbonization. SEM analysis revealed characteristic micro-irregularities of the Er:YAG-lased surface and the melted, resolidified appearance surrounded by major and microcracks of the CO2-lased surface. Histological analysis revealed minimal thermal alteration and structural degradation of the Er:YAG laser-irradiated cementum with an affected layer of approximately 20-mu m thickness, which partially consisted of two distinct affected layers. The CO2-lased cementum revealed multiple affected layers showing different structures/staining with approximately 140 mu m thickness. Er:YAG laser irradiation used with water cooling resulted in minimal cementum ablation and thermal changes with a characteristic microstructure of the superficial layer. In contrast, CO2 laser irradiation produced severely affected distinct multiple layers accompanied by melting and carbonization.
引用
收藏
页码:203 / 213
页数:11
相关论文
共 43 条
[1]
ULTRASTRUCTURAL OBSERVATIONS ON BACTERIAL INVASION IN CEMENTUM AND RADICULAR DENTIN OF PERIODONTALLY DISEASED HUMAN-TEETH [J].
ADRIAENS, PA ;
EDWARDS, CA ;
DEBOEVER, JA ;
LOESCHE, WJ .
JOURNAL OF PERIODONTOLOGY, 1988, 59 (08) :493-503
[2]
Lasers in nonsurgical periodontal therapy [J].
Aoki, A ;
Sasaki, KM ;
Watanabe, H ;
Ishikawa, I .
PERIODONTOLOGY 2000, 2004, 36 :59-97
[3]
Comparison between Er:YAG laser and conventional technique for root caries treatment in vitro [J].
Aoki, A ;
Ishikawa, I ;
Yamada, T ;
Otsuki, M ;
Watanabe, H ;
Tagami, J ;
Ando, Y ;
Yamamoto, H .
JOURNAL OF DENTAL RESEARCH, 1998, 77 (06) :1404-1414
[4]
IN-VITRO STUDIES ON LASER SCALING OF SUBGINGIVAL CALCULUS WITH AN ERBIUM-YAG LASER [J].
AOKI, A ;
ANDO, Y ;
WATANABE, H ;
ISHIKAWA, I .
JOURNAL OF PERIODONTOLOGY, 1994, 65 (12) :1097-1106
[5]
In vitro evaluation of Er:YAG laser scaling of subgingival calculus in comparison with ultrasonic scaling [J].
Aoki, A ;
Miura, M ;
Akiyama, F ;
Nakagawa, N ;
Tanaka, J ;
Oda, S ;
Watanabe, H ;
Ishikawa, I .
JOURNAL OF PERIODONTAL RESEARCH, 2000, 35 (05) :266-277
[6]
Effect of Er:YAG laser combined with rhPDGF-BB on attachment of cultured fibroblasts to periodontally involved root surfaces [J].
Belal, Mahmoud Helmy ;
Watanabe, Hisashi ;
Ichinose, Shizuko ;
Ishikawa, Isao .
JOURNAL OF PERIODONTOLOGY, 2007, 78 (07) :1329-1341
[7]
Cobb CM, 2002, J CLIN PERIODONTOL, V29, P6
[8]
Efficacy of subgingival calculus removal with Er:YAG laser compared to mechanical debridement:: an in situ study [J].
Eberhard, J ;
Ehlers, H ;
Falk, W ;
Açil, Y ;
Albers, HK ;
Jepsen, S .
JOURNAL OF CLINICAL PERIODONTOLOGY, 2003, 30 (06) :511-518
[9]
Eguro T, 1998, JPN J CONSERV DENT, V41, P582
[10]
Featherstone J D, 2000, Dent Clin North Am, V44, P955