The phytotoxin coronatine induces light-dependent reactive oxygen species in tomato seedlings

被引:65
作者
Ishiga, Yasuhiro [1 ]
Uppalapati, Srinivasa Rao [1 ,2 ]
Ishiga, Takako [1 ]
Elavarthi, Sathya [3 ]
Martin, Bjorn [3 ]
Bender, Carol L. [1 ]
机构
[1] Oklahoma State Univ, Dept Entomol & Plant Pathol, Noble Res Ctr 127, Stillwater, OK 74078 USA
[2] Samuel Roberts Noble Fdn Inc, Div Plant Biol, Ardmore, OK 73401 USA
[3] Oklahoma State Univ, Dept Plant & Soil Sci, Stillwater, OK 74078 USA
基金
美国国家科学基金会; 日本学术振兴会;
关键词
bacterial speck; chlorosis; coronatine; jasmonates; necrosis; Pseudomonas syringae pv. tomato; reactive oxygen species (ROS); tomato (Solanum lycopersicum); SYRINGAE PV. TOMATO; PHOTOSYNTHETIC ELECTRON-TRANSPORT; ARABIDOPSIS-THALIANA; SALICYLIC-ACID; CELL-DEATH; METHYL JASMONATE; HYPERSENSITIVE RESPONSE; SIGNALING PATHWAYS; BACTERIAL-BLIGHT; OXIDATIVE STRESS;
D O I
10.1111/j.1469-8137.2008.02639.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The phytotoxin coronatine (COR), which is produced by Pseudomonas syringae pv. tomato DC3000 (DC3000), has multiple roles in virulence that lead to chlorosis and a reduction in chlorophyll content. However, the physiological significance of COR-induced chlorosis in disease development is still largely unknown. Global expression analysis demonstrated that DC3000 and COR, but not the COR-defective mutant DB29, caused reduced expression of photosynthesis-related genes and result in a 1.5-to 2-fold reduction in maximum quantum efficiency of photosystem II (FV/FM). Tomato (Solanum lycopersicum) seedlings inoculated with DC3000 and incubated in a long daily photoperiod showed more necrosis than inoculated seedlings incubated in either dark or a short daily photoperiod. The accumulation of reactive oxygen species (ROS) was detected in cotyledons inoculated with either purified COR or DC3000 but not in tissues inoculated with DB29. Interestingly, COR-induced ROS accumulated only in light and was inhibited by 3-(3,4-dichlorophenyl)-1,1-dimethylurea and diphenylene iodonium, which function to inhibit electron transport from PSII. Furthermore, COR and DC3000 suppressed expression of the gene encoding the thylakoid Cu/Zn superoxide dismutase but not the cytosolic form of the same enzyme. In conclusion, these results demonstrate a role for COR-induced effects on photosynthetic machinery and ROS in modulating necrotic cell death during bacterial speck disease of tomato.
引用
收藏
页码:147 / 160
页数:14
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