An improved tetracycline-inducible expression vector for Staphylococcus aureus

被引：131

作者：

Corrigan, Rebecca M. ^{[1
]}

Foster, Timothy J. ^{[1
]}

机构：

[1] Univ Dublin Trinity Coll, Moyne Inst Prevent Med, Dept Microbiol, Dublin 2, Ireland

来源：

PLASMID | 2009年 / 61卷 / 02期

基金：

爱尔兰科学基金会;

关键词：

Expression vector; Surface protein SasG; Tetracycline induction; Controlled expression; SURFACE PROTEIN SASG; GENE-EXPRESSION; ADHERENCE; VIRULENCE;

D O I：

10.1016/j.plasmid.2008.10.001

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The tetracycline-inducible expression vector pALC2073 allowed high level expression of the cloned sasG gene but repression by uninduced cells was leaky. The -10 box of the tetR promoter was mutated to the Bacillus subtitlis consensus, which resulted in complete repression of SasG protein expression. Anhydrotetracycline at 1.28 mu g ml(-1) gave the same high level of induction that was obtained with pALC2073sasG using 160 ng ml(-1) tetracycline, the highest concentration that could be used without inhibiting bacterial growth. This variant of pALC2073 thus offers almost complete repression when uninduced and high levels of expression when induced. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：126 / 129

页数：4

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