Chitosan nanofiber scaffold enhances hepatocyte adhesion and function

被引：91

作者：

Chu, Xue-Hui ^{[1
]}

Shi, Xiao-Lei ^{[1
]}

Feng, Zhang-Qi ^{[2
]}

Gu, Zhong-Ze ^{[2
]}

Ding, Yi-Tao ^{[1
]}

机构：

[1] Nanjing Univ, Sch Med, Drum Tower Affiliated Hosp, Dept Hepatobiliary Surg, Nanjing 210008, Jiangsu, Peoples R China

[2] Southeast Univ, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2009年 / 31卷 / 03期

关键词：

Bioartificial liver; Chitosan; Hepatocyte; Nanofiber scaffold; CELL-ADHESION; BIOCOMPATIBILITY; REGENERATION;

D O I：

10.1007/s10529-008-9892-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

To enhance cell attachment and promote liver functions of hepatocytes cultured in bioreactors, a chitosan nanofiber scaffold was designed and prepared via electrospinning. Effects of the scaffold on hepatocyte adhesion, viability and function were then investigated. Data showed that hepatocytes on chitosan nanofiber scaffold exhibited better viability and tighter cell-substrate contact than cells on regular chitosan film. In addition, urea synthesis, albumin secretion and cytochrome P450 activity of hepatocytes on chitosan nanofiber scaffold were all 1.5 to 2 folds higher than the controls. Glycogen synthesis was also increased as compared with the controls. These results suggested the potential application of this chitosan nanofiber scaffold as a suitable substratum for hepatocyte culturing in bioreactors.

引用

页码：347 / 352

页数：6

共 13 条

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Leptin stimulates glucose transport and glycogen synthesis in C2C12 myotubes: Evidence for a PI3-kinase mediated effect [J].