Neutralizing antibodies inhibit axonal spread of herpes simplex virus type 1 to epidermal cells in vitro

被引:54
作者
Mikloska, Z [1 ]
Sanna, PP
Cunningham, AL
机构
[1] Westmead Hosp, Westmead Inst Hlth Res, Ctr Virus Res, Westmead, NSW 2145, Australia
[2] Univ Sydney, Westmead, NSW 2145, Australia
[3] Scripps Res Inst, Dept Neuropharmacol, La Jolla, CA 92037 USA
关键词
D O I
10.1128/JVI.73.7.5934-5944.1999
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The ability of antibodies to interfere with anterograde transmission of herpes simplex virus (HSV) from neuronal axons to the epidermis was investigated in an in vitro model consisting of human fetal dorsal root ganglia innervating autologous skin explants in a dual-chamber tissue culture system. The number and size of viral cytopathic plaques in epidermal cells after axonal transmission from HSV type 1 (HSV-1)-infected dorsal root ganglionic neurons were significantly reduced by addition to the outer chamber of neutralizing polyclonal human sera to HSV-1, of a human recombinant monoclonal group Ib antibody to glycoprotein D (gD), and of rabbit sera to HSV-1 gB and go but not by rabbit anti-gE or anti-gG. A similar pattern of inhibition of direct infection of epidermal cells by these antibodies was observed. High concentrations of the monoclonal anti-go reduced transmission by 90%, Rabbit anti-gB was not taken up into neurons, and human anti-go did not influence spread of HSV in the dorsal root ganglia or axonal transport of HSV antigens when applied to individual dissociated neurons. These results suggest that anti-go and -gB antibodies interfere with axonal spread of HSV-1, possibly by neutralizing RSV during transmission across an intercellular gap between axonal termini and epidermal cells, and thus contribute to control of HSV spread and shedding. Therefore, selected human monoclonal antibodies to protective epitopes might even be effective in preventing epidermis-to-neuron transmission during primary HSV infection, especially neonatal infection.
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页码:5934 / 5944
页数:11
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